Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 24, Number 9—September 2018
Dispatch

Association of Batai Virus Infection and Encephalitis in Harbor Seals, Germany, 2016

Wendy K. Jo1, Vanessa M. Pfankuche1, Annika Lehmbecker, Byron Martina, Ana Rubio-Garcia, Stefanie Becker, Jochen Kruppa, Klaus Jung, Daniela Klotz, Julia Metzger, Martin Ludlow, Wolfgang Baumgärtner, Erhard van der Vries2, and Albert OsterhausComments to Author 
Author affiliations: University of Veterinary Medicine Hannover, Hannover, Germany (W.K. Jo, V.M. Pfankuche, A. Lehmbecker, S. Becker, J. Kruppa, K. Jung, D. Klotz, J. Metzger, M. Ludlow, W. Baumgärtner, E. van der Vries, A. Osterhaus); Center for Systems Neuroscience, Hannover (W.K. Jo, V.M. Pfankuche, W. Baumgärtner, A. Osterhaus); Artemis One Health, Delft, the Netherlands (B. Martina, A. Osterhaus); Seal Centre, Pieterburen, the Netherlands (A. Rubio-Garcia)

Main Article

Figure 2

Bayesian phylogeny trees based on full-genome coding region sequences of small, medium, and large RNA segments of Batai virus and comparison viruses. A) Small RNA segments (69–770 bp). Bunyamwera virus (GenBank accession no. D00353) was used as the outgroup. B) Medium RNA segments (42–4,346 bp). Bunyamwera virus (GenBank accession no. M11852) was used as the outgroup. C) Large RNA segments (49–6,762 bp). Bunyamwera virus (GenBank accession no. X14383) was used as the outgroup. Bold indicates vir

Figure 2. Bayesian phylogeny trees based on full-genome coding region sequences of small, medium, and large RNA segments of Batai virus and comparison viruses. A) Small RNA segments (69–770 bp). Bunyamwera virus (GenBank accession no. D00353) was used as the outgroup. B) Medium RNA segments (42–4,346 bp). Bunyamwera virus (GenBank accession no. M11852) was used as the outgroup. C) Large RNA segments (49–6,762 bp). Bunyamwera virus (GenBank accession no. X14383) was used as the outgroup. Bold indicates virus isolated in this study. Analysis was performed for 1 million generations and sampled every 100 steps. The first 25% of samples were discarded as burn-in according to MrBayes (11). Hasegawa-Kishino-Yano nucleotide substitution model was selected as best-fit model according to Bayesian information criteria. Numbers at the nodes indicate posterior probabilities percentage. GenBank accession numbers are provided for comparison isolates; accession nos. of the isolated Batai virus strain PV424/DE-2016 are small, MH299972; medium, MH299973; large, MH299974. Scale bars indicate nucleotide substitutions per site.

Main Article

References
  1. Elliott  RM. Orthobunyaviruses: recent genetic and structural insights. Nat Rev Microbiol. 2014;12:673–85. DOIPubMedGoogle Scholar
  2. Yanase  T, Kato  T, Yamakawa  M, Takayoshi  K, Nakamura  K, Kokuba  T, et al. Genetic characterization of Batai virus indicates a genomic reassortment between orthobunyaviruses in nature. Arch Virol. 2006;151:2253–60. DOIPubMedGoogle Scholar
  3. Hubálek  Z. Mosquito-borne viruses in Europe. Parasitol Res. 2008;103(Suppl 1):S29–43. DOIPubMedGoogle Scholar
  4. Nashed  NW, Olson  JG, el-Tigani  A. Isolation of Batai virus (Bunyaviridae:Bunyavirus) from the blood of suspected malaria patients in Sudan. Am J Trop Med Hyg. 1993;48:676–81. DOIPubMedGoogle Scholar
  5. Jöst  H, Bialonski  A, Schmetz  C, Günther  S, Becker  N, Schmidt-Chanasit  J. Isolation and phylogenetic analysis of Batai virus, Germany. Am J Trop Med Hyg. 2011;84:241–3. DOIPubMedGoogle Scholar
  6. Hofmann  M, Wiethölter  A, Blaha  I, Jöst  H, Heinemann  P, Lehmann  M, et al. Surveillance of Batai virus in bovines from Germany. Clin Vaccine Immunol. 2015;22:672–3. DOIPubMedGoogle Scholar
  7. Lempp  C, Jungwirth  N, Grilo  ML, Reckendorf  A, Ulrich  A, van Neer  A, et al. Pathological findings in the red fox (Vulpes vulpes), stone marten (Martes foina) and raccoon dog (Nyctereutes procyonoides), with special emphasis on infectious and zoonotic agents in Northern Germany. PLoS One. 2017;12:e0175469. DOIPubMedGoogle Scholar
  8. Uhde  AK, Lehmbecker  A, Baumgärtner  W, Spitzbarth  I. Evaluation of a panel of antibodies for the immunohistochemical identification of immune cells in paraffin-embedded lymphoid tissues of new- and old-world camelids. Vet Immunol Immunopathol. 2017;184:42–53. DOIPubMedGoogle Scholar
  9. Allander  T, Emerson  SU, Engle  RE, Purcell  RH, Bukh  J. A virus discovery method incorporating DNase treatment and its application to the identification of two bovine parvovirus species. Proc Natl Acad Sci U S A. 2001;98:11609–14. DOIPubMedGoogle Scholar
  10. Endoh  D, Mizutani  T, Kirisawa  R, Maki  Y, Saito  H, Kon  Y, et al. Species-independent detection of RNA virus by representational difference analysis using non-ribosomal hexanucleotides for reverse transcription. Nucleic Acids Res. 2005;33:e65. DOIPubMedGoogle Scholar
  11. Huelsenbeck  JP, Ronquist  F. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001;17:754–5. DOIPubMedGoogle Scholar
  12. Pfankuche  VM, Bodewes  R, Hahn  K, Puff  C, Beineke  A, Habierski  A, et al. Porcine bocavirus infection associated with encephalomyelitis in a Pig, Germany. Emerg Infect Dis. 2016;22:1310–2. DOIPubMedGoogle Scholar
  13. Bodewes  R, Zohari  S, Krog  JS, Hall  MD, Harder  TC, Bestebroer  TM, et al. Spatiotemporal analysis of the genetic diversity of seal influenza A(H10N7) virus, northwestern Europe. J Virol. 2016;90:4269–77. DOIPubMedGoogle Scholar
  14. Ludlow  M, Kortekaas  J, Herden  C, Hoffmann  B, Tappe  D, Trebst  C, et al. Neurotropic virus infections as the cause of immediate and delayed neuropathology. Acta Neuropathol. 2016;131:159–84. DOIPubMedGoogle Scholar
  15. Tauro  LB, Rivarola  ME, Lucca  E, Mariño  B, Mazzini  R, Cardoso  JF, et al. First isolation of Bunyamwera virus (Bunyaviridae family) from horses with neurological disease and an abortion in Argentina. Vet J. 2015;206:111–4. DOIPubMedGoogle Scholar

Main Article

1These authors contributed equally to this article.

2Current affiliation: Utrecht University, Utrecht, the Netherlands.

Page created: August 15, 2018
Page updated: August 15, 2018
Page reviewed: August 15, 2018
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external