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Volume 25, Number 10—October 2019
Research

Comparison of Serologic Assays for Middle East Respiratory Syndrome Coronavirus

Ruth Harvey, Giada MattiuzzoComments to Author , Mark Hassall, Andrea Sieberg, Marcel A. Müller, Christian Drosten, Peter Rigsby, Christopher J. Oxenford, and study participants
Author affiliations: National Institute for Biological Standards and Control—MHRA, Potters Bar, UK (R. Harvey, G. Mattiuzzo, M. Hassall, P. Rigsby); Charité-Universitätsmedizin Berlin, Berlin, Germany (A. Sieberg, M.A. Müller, C. Drosten); German Centre for Infection Research, Berlin (M.A. Müller, C. Drosten); World Health Organization, Lyon, France (C.J. Oxenford).

Main Article

Table 1

Samples used in study of serologic assays for MERS-CoV*

No. Name Description Expected result
1
 Korea 5
 Single plasma from laboratory-confirmed MERS patient
 Positive
2
 Tc Bovine NC
 Purified IgG from transchromosomal bovine, negative control
 Negative
3
 WHO/B
 Negative control serum, high titer for other CoV
 Negative
4
 Tc Bovine SAB 300
 Purified IgG from transchromosomal bovine, antigen whole virus
 Positive
5
 Korea 2
 Single plasma from laboratory-confirmed MERS patient
 Positive
6
 WHO/G
 Negative control serum, high titer for other CoV
 Negative
7
 WHO/A
 Negative control serum, high titer for other CoV
 Negative
8
 WHO/D
 Negative control serum, high titer for other CoV
 Negative
9
 Korea 3
 Single plasma from laboratory-confirmed MERS patient
 Positive
10
 Tc Bovine SAB 301
 Purified IgG from transchromosomal bovine, antigen spike protein
 Positive
11
 Korea 1
 Single plasma from laboratory-confirmed MERS patient
 Positive
12
 Korea 4
 Single plasma from laboratory-confirmed MERS patient
 Positive
13
 WHO/F
 Negative control serum, high titer for other CoV
 Negative
14
 Pool C (low)
 Pooled serum samples from laboratory-confirmed MERS patients
 Positive
15
 WHO/C
 Negative control serum, high titer for other CoV
 Negative
16
 Pool A (high)
 Pooled serum samples from laboratory-confirmed MERS patients
 Positive
17
 WHO/E
 Negative control serum, high titer for other CoV
 Negative
18 Pool B (medium) Pooled serum samples from laboratory-confirmed MERS patients Positive

*All samples were submitted as liquid in screw-cap tubes. CoV, coronavirus; MERS, Middle East respiratory syndrome; Tc, transchromosomal; WHO, World Health Organization.

Main Article

1Study participants who contributed data are listed at the end of this article.

Page created: September 17, 2019
Page updated: September 17, 2019
Page reviewed: September 17, 2019
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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