Figure 2. Phylogenetic relationships between bat influenza A viruses from Brazil and reference viruses. Phylogenetic trees show comparison of the 8 segments of representative influenza A virus genomes (PB2, PB1, PA, HA/HL, NP, NA/NL, M, NS) with A/great fruit-eating bat/Brazil/2301/2012 (HL18NL11a; GenBank accession nos. MH682200–7) and A/great fruit-eating bat/Brazil/2344/2012 (HL18NL11b; GenBank accession nos. MH682208–15), shown in red. Maximum-likelihood trees were inferred using a general time-reversible substitution model with a gamma distribution and invariant sites. Black dots represent bootstrap values >75% (1,000 replicates). Trees were generally rooted using influenza B/Lee/1940 (GenBank accession nos. DQ792894–901) (data not shown). Trees were constructed by using MEGA 6.0 (http://www.megasoftware.net). HA, hemagglutinin; M, matrix; NA, neuraminidase; NS1, nonstructural protein 1; NP, nucleoprotein; PA, polymerase acidic; PB, polymerase basic. Scale bars indicate nucleotide substitutions per site.