Volume 25, Number 5—May 2019
Research
Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues
Jennifer Chua1
, Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. Cote
, Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. Cote
Table 4
Routes of infection and delivered doses of Select Agents used to infect guinea pigs and rabbits for tissue collection in study of inactivation of bacterial Tier 1 Select Agents
| Bacteria | Route of infection | Delivered dose, CFU | Animal model | Time of collection | Tissue collected |
|---|---|---|---|---|---|
| Bacillus anthracis Ames (spores) | Intradermal | 2.7 × 108 | Guinea pigs-Hartley | 2 h after challenge | Skin |
| Inhalational and/or intramuscular, respiratory | 4.9 × 106 and 1.0 × 103 respiratory | Guinea pigs-Strain 13 | Late-stage disease | Lung, liver, spleen | |
| Inhalational |
1.0 × 107 |
Rabbits-New Zealand White |
24 h after challenge |
Lung |
|
| Burkholderia pseudomallei 1026b |
Intraperitoneal |
9.0 × 102 |
Guinea pigs-Strain 13 |
Late-stage disease |
Lung, liver, spleen |
| Francisella tularensis Schu S4 |
Subcutaneous |
8.6 × 102 |
Guinea pigs-Strain 13 |
Late-stage disease |
Lung, liver spleen |
| Yersina pestis CO92 | Subcutaneous | 7.0 × 104 | Guinea pigs-Strain 13 | Late-stage disease | Lung, liver, spleen |
1These authors contributed equally to this article.
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