Volume 25, Number 5—May 2019
Research
Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues
Jennifer Chua1
, Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. Cote
, Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. Cote
Table 8
Formaldehyde fixation of infected lung tissue from rabbits exposed to aerosolized Bacillus anthracis Ames spores in study of inactivation of bacterial Tier I Select Agents*
| Rabbit no. | Tissue weight, g | Tissue size, mm | Total CFU recovered/g | Heat-resistant CFU recovered/g | Time of inactivation |
Positive control | |
|---|---|---|---|---|---|---|---|
| 13 d | 20 d | ||||||
| 1 | 0.72 | 10 × 10 × 10 | 1.2 × 107 | 1.5 × 106 | –/–† | ND | +/+ |
| 2 | 0.74 | 10 × 10 × 10 | 4.4 × 105 | 4.3 × 105 | –/–† | ND | +/+ |
| 3 | 0.71/0.75 | 10 × 10 × 10 | 2.6 × 106 | 1.0 × 106 | +/+† | –/–† | +/+ |
*Infected lung tissues from 3 rabbits were fixed for 13 or 20 d in 4% paraformaldehyde, rinsed for 30 min in phosphate-buffered saline, and then homogenized. Listed are the tissue weight, size, and CFU recovered. +/– indicates presence and/or absence of growth in broth/on plate. ND, not done.
†50% of sample tested.
1These authors contributed equally to this article.
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