Volume 25, Number 5—May 2019
Research
Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues
Jennifer Chua1
, Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. Cote
, Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. Cote
Table 1
Formaldehyde fixation of Bacillus anthracis Ames–infected skin tissue from guinea pigs in study of inactivation of bacterial Tier 1 Select Agents*
| Fixation time, d, no. samples | Maximum tissue weight, g | Maximum tissue size, mm | Total CFU recovered/g | Sample inactivation† | Positive control |
|---|---|---|---|---|---|
| 7, 3 | 2.04 | 20 × 30 × 3 | 6.15 × 107 | +/+‡ | +/+ |
| 14, 3 | 1.94 | 23 × 28 × 3 | 5.41 × 107 | –/– | +/+ |
| 21, 3 | 2.55 | 28 × 28 × 3 | 4.86 × 107 | –/– | +/+ |
*Infected skin tissues from 3 guinea pigs were fixed for 7, 14, or 21 d in 4% paraformaldehyde, rinsed for 30 min in phosphate-buffered saline, and then homogenized. The maximum tissue weight, size, and CFU recovered per group are listed. +/– indicates presence or absence of growth in broth/on plates.
†100% of sample tested.
‡Of 3 samples, 1 was positive for growth.
1These authors contributed equally to this article.
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