Volume 25, Number 5—May 2019
Research
Infectious Dose of African Swine Fever Virus When Consumed Naturally in Liquid or Feed
Megan C. Niederwerder
, Ana M.M. Stoian, Raymond R.R. Rowland, Steve S. Dritz, Vlad Petrovan, Laura A. Constance, Jordan T. Gebhardt, Matthew Olcha, Cassandra K. Jones, Jason C. Woodworth, Ying Fang, Jia Liang, and Trevor J. Hefley
, Ana M.M. Stoian, Raymond R.R. Rowland, Steve S. Dritz, Vlad Petrovan, Laura A. Constance, Jordan T. Gebhardt, Matthew Olcha, Cassandra K. Jones, Jason C. Woodworth, Ying Fang, Jia Liang, and Trevor J. Hefley
Table 1
Replicates of pigs orally exposed to ASFV in liquid or feed based on a sequential adaptive experimental design to determine the infectious dose of ASFV when consumed naturally*
| Dose ASFV, TCID50 |
Liquid media replicates, no. tested (no. positive) |
Plant-based feed replicates, no. tested (no. positive) |
||||||||||||||
| 1 |
2 |
3 |
4 |
5 |
6 |
7 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
|||
| 100 | – | – | – | – | 3 (3) | – | 5 (0) | – | – | – | – | – | – | – | ||
| 101 | – | – | 5 (3) | 5 (1)† | – | – | – | – | – | – | – | – | – | – | ||
| 102 | – | 4 (2) | – | – | 2 (2) | 2 (2) | – | – | – | – | – | – | – | – | ||
| 103 | 5 (5) | 1 (0) | – | – | – | – | – | – | 5 (0) | – | – | – | – | – | ||
| 104 | – | – | – | – | – | 3 (3) | – | 5 (2) | – | – | – | – | – | – | ||
| 105 | – | – | – | – | – | – | – | – | – | 5 (2) | 5 (2)† | – | – | – | ||
| 106 | – | – | – | – | – | – | – | – | – | – | – | 3 (0) | – | 5 (2) | ||
| 107 | – | – | – | – | – | – | – | – | – | – | – | 2 (0) | 3 (2) | – | ||
| 108 | – | – | – | – | – | – | – | – | – | – | – | – | 2 (1) | – | ||
*Data are shown for the 5 infected pigs. In each replicate, 1 negative control pig was present. ASFV, African swine fever virus; TCID50, 50% tissue culture infectious dose; –, no pigs tested.
†One pig in each of these replicates died before 5 days postinoculation for causes other than ASF and was eliminated from the data analysis.
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Page updated: April 18, 2019
Page reviewed: April 18, 2019
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