Figure 2. Granulibacter bethesdensis colonies and pathology results after inoculation on mouse models. A, B) CSF on chocolate agar for 4 days showed slowly growing brown-yellow, shiny colonies 2–4 mm in diameter. Full 16S RNA sequencing (≈1,500 bp) led to identification of G. bethesdensis (with 99.7% match). C) Survival of gp91/p47 KO mice after inoculation of different G. bethesdensis strains. D) Pathology images of gp91 KO mouse lung after CSF strain infection. E) Pathology images of gp91 KO mouse lungs after type strain infection. F) Quantification of G. bethesdensis strains in spleens of gp91/p47 KO mice after inoculation. CSF, cerebrospinal fluid; type, National Institutes of Health type strain; WT, wild type.