Volume 25, Number 5—May 2019
Novel Picornavirus in Lambs with Severe Encephalomyelitis
|Year||Signalment (case reference)||Case information||RT-PCR†||OvPV reads, no. (%)‡||Reference coverage§||Nucl. seq. identity§|
||3-week-old mule lamb (1454/98)
||Clinical signs of head tilt, rolling eyes and star gazing, and rapid onset of ataxia, recumbency, and paddling; no response to antimicrobial drugs, multivitamins including B1. Negative for Louping ill virus antibodies in cerebrospinal fluid and serum.
||2–4-week-old milking breed lamb (960/00)
||One of ≈12 lambs fed artificial colostrum (snatched at birth to prevent transmission of maedi-visna virus) that developed neurologic signs at ≈2 weeks of age, first weak in forelimbs, then hindlimbs, and by the second day, tremor and incoordination.
||Two 7–8-day-old triplet lambs (E1028/04 and E1029/04)
||Triplets fed proprietary powdered colostrum because the ewe had insufficient milk. Signs of fine tremor progressing to recumbency; the third triplet developed similar neurologic signs at 3 weeks of age (not submitted for investigation).
||Cq 32; positive
||747,777 (3.02); 238,627 (1.11)
||2-week-old Texel X Swaledale (S313-04-08-1)
||Neurologic signs not otherwise specified.
||1-week-old mule X female lamb (S78-04-10-1)
||Approximately 25 lambs have been similarly affected. Portions of fixed juvenile ovine brain were submitted from field postmortem examination with history of suspected neurologic disease before euthanasia.
|2014||Breed and age not known (S247-04-14)||Preweaned lamb with neurologic signs. Field postmortem samples submitted with clinical history of “suspect swayback,” thus likely clinical signs of ataxia and paresis.||Cq 34||NP||NP||NP|
*Cq, PCR quantification cycle; NP, not performed; nucl. seq., nucleotide sequence; OvPV, ovine picornavirus; RT-PCR, reverse transcription PCR; qRT-PCR, quantitative RT-PCR.
†RT-PCR was performed as prescreening for sample selection for high-throughput sequencing. Where feasible, Cq values of the qRT-PCR are indicated; positive means positive in semi-nested PCR.
‡Number (proportion) of OvPV reads in the complete high-throughput sequencing dataset.
§Genome coverage and nucleotide sequence identity of the full genome or several contigs in relation to the OvPV reference genome strain OvPV/Wales/2017/S014175 (International Nucleotide Sequence Data Collaboration accession no. LR216008).