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Volume 25, Number 6—June 2019

Pertactin-Negative and Filamentous Hemagglutinin-Negative Bordetella pertussis, Australia, 2013–2017

Zheng Xu, Sophie Octavia, Laurence Don Wai Luu, Michael Payne, Verlaine Timms, Chin Yen Tay, Anthony D. Keil, Vitali Sintchenko, Nicole Guiso, and Ruiting LanComments to Author 
Author affiliations: University of New South Wales, Sydney, New South Wales, Australia (Z. Xu, S. Octavia, L.D.W. Luu, M. Payne, R. Lan); New South Wales Health Pathology and Westmead Hospital, Sydney (V. Timms, V. Sintchenko); The University of Sydney, Sydney (V. Timms, V. Sintchenko); University of Western Australia, Perth, Western Australia, Australia (C.Y. Tay); Perth Children’s Hospital, Perth (A.D. Keil); Institut Pasteur, Paris, France (N. Guiso)

Main Article


Mechanisms of pertactin deficiency and characteristics of Bordetella pertussis isolates from pertussis epidemics, Australia, 2013–2017*

Prn deficiency mechanism Position in prn prn allele type State (no. of isolates) Year (no. isolates) References
IS481F 1613 prn2 Western Australia (32) 2013 (13) (1)
New South Wales (10) 2014 (5)
2015 (11)
2016 (9)

2017 (4)

IS481R 1613 prn2 Western Australia (12) 2013 (6) (1)
New South Wales (5) 2014 (5)

2015 (4)

IS481F 1598 prn3 Western Australia (3) 2013 (1) This study

2014 (2)

IS1002R 1613 prn2 Western Australia (4) 2013 (2) (1)
2016 (1)

2017 (1)

Deletion −297 to 1325 Not determined‡ Western Australia (2) 2014 (1) (8), newly found in Australia

2015 (1)

Stop codon
Western Australia (1)
2014 (1)
(10), newly found in Australia
Deletion 2020–2023 prn2 Western Australia (1) 2013 (1) This study

*F/R denotes IS insertion orientation relative to prn. F, forward; IS, insertion sequence; Prn, pertactin; R, reverse.
†The nucleotide positions are relative to the initiation codon (ATG) of the prn in Tohama I.
prn allele type was not determinable because the repeat regions that define prn allele type was deleted in this mechanism.

Main Article

  1. Lam  C, Octavia  S, Ricafort  L, Sintchenko  V, Gilbert  GL, Wood  N, et al. Rapid increase in pertactin-deficient Bordetella pertussis isolates, Australia. Emerg Infect Dis. 2014;20:62633. DOIPubMedGoogle Scholar
  2. Octavia  S, Sintchenko  V, Gilbert  GL, Lawrence  A, Keil  AD, Hogg  G, et al. Newly emerging clones of Bordetella pertussis carrying prn2 and ptxP3 alleles implicated in Australian pertussis epidemic in 2008-2010. J Infect Dis. 2012;205:12204. DOIPubMedGoogle Scholar
  3. Octavia  S, Maharjan  RP, Sintchenko  V, Stevenson  G, Reeves  PR, Gilbert  GL, et al. Insight into evolution of Bordetella pertussis from comparative genomic analysis: evidence of vaccine-driven selection. Mol Biol Evol. 2011;28:70715. DOIPubMedGoogle Scholar
  4. Safarchi  A, Octavia  S, Wu  SZ, Kaur  S, Sintchenko  V, Gilbert  GL, et al. Genomic dissection of Australian Bordetella pertussis isolates from the 2008-2012 epidemic. J Infect. 2016;72:46877. DOIPubMedGoogle Scholar
  5. Williamson  P, Matthews  R. Epitope mapping the Fim2 and Fim3 proteins of Bordetella pertussis with sera from patients infected with or vaccinated against whooping cough. FEMS Immunol Med Microbiol. 1996;13:16978.PubMedGoogle Scholar
  6. Choi  Y, Sims  GE, Murphy  S, Miller  JR, Chan  AP. Predicting the functional effect of amino acid substitutions and indels. PLoS One. 2012;7:e46688. DOIPubMedGoogle Scholar
  7. Weigand  MR, Peng  Y, Cassiday  PK, Loparev  VN, Johnson  T, Juieng  P, et al. Complete genome sequences of Bordetella pertussis isolates with novel pertactin-deficient deletions. Genome Announc. 2017;5:e0097317. DOIPubMedGoogle Scholar
  8. Pawloski  LC, Queenan  AM, Cassiday  PK, Lynch  AS, Harrison  MJ, Shang  W, et al. Prevalence and molecular characterization of pertactin-deficient Bordetella pertussis in the United States. Clin Vaccine Immunol. 2014;21:11925. DOIPubMedGoogle Scholar
  9. Otsuka  N, Han  HJ, Toyoizumi-Ajisaka  H, Nakamura  Y, Arakawa  Y, Shibayama  K, et al. Prevalence and genetic characterization of pertactin-deficient Bordetella pertussis in Japan. PLoS One. 2012;7:e31985. DOIPubMedGoogle Scholar
  10. Weigand  MR, Peng  Y, Loparev  V, Batra  D, Bowden  KE, Burroughs  M, et al. The history of Bordetella pertussis genome evolution includes structural rearrangement. J Bacteriol. 2017;199:e0080616. DOIPubMedGoogle Scholar
  11. Bart  MJ, Harris  SR, Advani  A, Arakawa  Y, Bottero  D, Bouchez  V, et al. Global population structure and evolution of Bordetella pertussis and their relationship with vaccination. MBio. 2014;5:e01074. DOIPubMedGoogle Scholar
  12. Luu  LDW, Octavia  S, Zhong  L, Raftery  M, Sintchenko  V, Lan  R. Characterisation of the Bordetella pertussis secretome under different media. J Proteomics. 2017;158:4351. DOIPubMedGoogle Scholar
  13. Hiramatsu  Y, Miyaji  Y, Otsuka  N, Arakawa  Y, Shibayama  K, Kamachi  K. Significant decrease in pertactin-deficient Bordetella pertussis isolates, Japan. Emerg Infect Dis. 2017;23:699701. DOIPubMedGoogle Scholar

Main Article

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Page updated: May 20, 2019
Page reviewed: May 20, 2019
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