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Volume 25, Number 6—June 2019
Research

Mass Die-Off of Saiga Antelopes, Kazakhstan, 2015

Sasan Fereidouni, Graham L. Freimanis, Mukhit Orynbayev, Paolo Ribeca, John Flannery, Donald P. King, Steffen Zuther, Martin Beer, Dirk Höper, Aidyn Kydyrmanov, Kobey Karamendin, and Richard KockComments to Author 
Author affiliations: University of Veterinary Medicine Vienna, Vienna, Austria (S. Fereidouni); The Pirbright Institute, Pirbright, UK (G.L. Freimanis, P. Ribeca, J. Flannery, D.P. King); Research Institute for Biological Safety Problems, Otar, Kazakhstan (M. Orynbayev); Association for the Conservation of Biodiversity of Kazakhstan, Astana, Kazakhstan (S. Zuther); Frankfurt Zoological Society, Frankfurt, Germany (S. Zuther); Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (M. Beer, D. Höper); Institute of Microbiology and Virology, Almaty, Kazakhstan (A. Kydrymanov, K. Karamendin); Royal Veterinary College, London, UK (R. Kock)

Main Article

Table 5

Summary of the most relevant results obtained by RIEMS analyses of the datasets (sequenced from shotgun libraries generated from random primed cDNA) used in an investigation of a mass die-off of saiga antelopes, Kazakhstan, 2015

Organism No. (%) reads
Sample 2* (lib01416) Sample 5* (lib01417) Sample 7 (lib01418) Sample 8 (lib01419)
Input reads 411,640 (100) 376,210 (100) 372,387 (100) 354,958 (100)
Quality filtered reads† 12,786 (3.1) 10,793 (2.9) 11,559 (3.1) 10,895 (3.1)
Unclassified reads† 1,776 (0.43) 1,520 (0.40) 1,626 (0.44) 1,494 (0.42)
Classified reads† 397,078 (96.5) 363,897 (96.7) 359,202 (96.5) 342,569 (96.5)
Host‡ 64,618 (16.3) 4,770 (1.3) 3,414 (1.0) 4,784 (1.4)
Pasteurellaceae‡ 317,009 (79.8) 345,893 (95.1) 339,484 (94.5) 324,770 (94.8)

*Samples 2 and 5 were also tested by using high-throughput sequencing at the Pirbright Institute.
†Percentage is of the number of input reads.
‡Percentage is of the number of classified reads.

Main Article

Page created: May 20, 2019
Page updated: May 20, 2019
Page reviewed: May 20, 2019
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