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Volume 25, Number 6—June 2019
Dispatch

New Delhi Metallo-β-Lactamase 5–Producing Klebsiella pneumoniae Sequence Type 258, Southwest China, 2017

Xin Zhang1, Weimin Wan1, Hua Yu1, Min Wang, Haifang Zhang, Jingnan Lv, Yi-Wei Tang, Barry N. Kreiswirth, Hong DuComments to Author , and Liang Chen
Author affiliations: Sichuan Academy of Medical Science and Sichuan Provincial People’s Hospital, Sichuan (X. Zhang, H. Yu); Memorial Sloan Kettering Cancer Center, New York, New York, USA (X. Zhang, Y.-W. Tang); The Second Affiliated Hospital of Soochow University, Suzhou, China (W. Wan, M. Wang, H. Zhang, J. Lv, H. Du); Rutgers University, Newark, New Jersey, USA (B.N. Kreiswirth, L. Chen)

Main Article

Figure 2

Phylogenetic analysis of KPC-producing and NDM-5–producing CG258 Klebsiella pneumoniae strains from China, 2017, and reference strains. A) Core SNP phylogenetic analysis of 76 global CG258 (ST258 and ST11) and 2 ST258 strains from China. Lane 1, Bla_carb; lane 2, wzi (cps); lane 3, integrative and conjugative element Kp258.2; lane 4, marR; lane 5, ompK35 gene (guanine insertion at nt position 121). The maximum-likelihood tree was rooted by using ST11 strains. Bootstrap values >90% are indicat

Figure 2. Phylogenetic analysis of KPC-producing and NDM-5–producing CG258 Klebsiella pneumoniae strains from China, 2017, and reference strains. A) Core SNP phylogenetic analysis of 76 global CG258 (ST258 and ST11) and 2 ST258 strains from China. Lane 1, Bla_carb; lane 2, wzi (cps); lane 3, integrative and conjugative element Kp258.2; lane 4, marR; lane 5, ompK35 gene (guanine insertion at nt position 121). The maximum-likelihood tree was rooted by using ST11 strains. Bootstrap values >90% are indicated as gray triangles at branch points. Sizes are proportion to values. Scale bar indicates nucleotide substitutions per site. B) Updated hypothesis of the molecular evolution of carbapenem-resistant K. pneumoniae ST258 (9). Bla, β-lactamase; Carb, carbapenemase; cps, capsular polysaccharide gene; ICE, integrative and conjugative element; ins, insertion; KPC, Klebsiella pneumoniae carbapenemase; MarR, transcriptional regulator protein of the multiple antimicrobial drug resistance repressor family; NDM, New Delhi metallo-β-lactamase; ompK35, outer membrane protein K35; OXA, oxacillinase; pKPC, plasmid carrying KPC; ST, sequence type; VIM-1, Verona integron–encoded metallo- β-lactamase 1; wzi, surface assembly of capsule gene.

Main Article

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1These authors contributed equally to this article.

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