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Volume 26, Number 1—January 2020
Research

High Pathogenicity of Nipah Virus from Pteropus lylei Fruit Bats, Cambodia

Maria Gaudino, Noémie Aurine, Claire Dumont, Julien Fouret, Marion Ferren, Cyrille Mathieu, Olivier Reynard, Viktor E. Volchkov, Catherine Legras-Lachuer, Marie-Claude Georges-Courbot, and Branka HorvatComments to Author 
Author affiliations: Centre International de Recherche en Infectiologie, CIRI, INSERM U1111, CNRS, UMR5308, Univ Lyon, University Claude Bernard Lyon 1, École Normale Supérieure de Lyon, Lyon, France (M. Gaudino, N. Aurine, C. Dumont, J. Fouret, M. Ferren, C. Mathieu, O. Reynard, V.E. Volchkov, M.-C. Georges-Courbot, B. Horvat); ViroScan 3D, Trévoux, France (J. Fouret, C. Legras-Lachuer); University Claude Bernard Lyon 1, LEM, UMR5557, CNRS, INRA, VetAgro Sup, Lyon (C. Legras-Lachuer); Unité de Biologie des Infections Virales Emergentes, Institut Pasteur, INSERM P4, Jean Mérieux, Lyon (M.-C. Georges-Courbot)

Main Article

Figure 4

Replication of NiVs CSUR381 (Cambodia 2003 isolate), UMMC1 (NiV-Malaysia isolate), and SPB200401066 (NiV-Bangladesh isolate) in Vero, HPMEC, NCI-H358 (human bronchioalveolar cells), and PATGV1.12 (bat cells). A) Real-time reverse transcription PCR titer. Cells were infected at a multiplicity of infection of 0.3, and the production of the nucleocapsid gene was measured. Significance was measured by 2-way analysis of variance. B) Kinetics of infectious virus particle production in supernatant meas

Figure 4. Replication of NiVs CSUR381 (Cambodia 2003 isolate), UMMC1 (NiV-Malaysia isolate), and SPB200401066 (NiV-Bangladesh isolate) in Vero, HPMEC, NCI-H358 (human bronchioalveolar cells), and PATGV1.12 (bat cells). A) Real-time reverse transcription PCR titer. Cells were infected at a multiplicity of infection of 0.3, and the production of the nucleocapsid gene was measured. Significance was measured by 2-way analysis of variance. B) Kinetics of infectious virus particle production in supernatant measured by Vero plaque assay. The average of 2 independent experiments is presented. C–E) Cytopathic effect of observed by light microscopy 48 h after infection with the NiV-Malaysia isolate (C), the NiV-Bangladesh isolate (D), and CSUR381 (E). Giant multinucleated cells are indicated with arrowheads. Scale bar indicates 25 µm. HPMEC, human pulmonary microvascular endothelial cell; NiV, Nipah virus. **p<0.01; ***p<0.001.

Main Article

Page created: December 18, 2019
Page updated: December 18, 2019
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