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Volume 26, Number 10—October 2020

Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCR

So Yeon Kim1, Jaehyeon Lee1, Heungsup Sung, Hyukmin Lee, Myung Guk Han, Cheon Kwon Yoo, Sang Won Lee2, and Ki Ho Hong2Comments to Author 
Author affiliations: National Medical Center, Seoul, South Korea (S.Y. Kim); Jeonbuk National University Medical School and Hospital, Jeonju, South Korea (J. Lee); Asan Medical Center and University of Ulsan College of Medicine, Seoul (H. Sung); Yonsei University College of Medicine, Seoul (H. Lee); Korea Centers for Disease Control and Prevention, Cheongju (M.G. Han, S.W. Lee, C.K. Yoo); Seoul Medical Center, Seoul (K.H. Hong)

Main Article


Test performance of pooled specimens compared with individual specimens for severe acute respiratory syndrome, virus 2

No. specimens in pool Amplification in E or RdRp gene, % No amplifications Sensitivity of pools, % (95% CI) Cumulative sensitivity, %*
2 100 0 100 (96–100) 100
4 100 0 100 (96–100) 100
6 100 0 100 (96–100) 100
8 97 3 97 (92–99) 97
10 99 1 99 (95–100) 96
16 96 4 96 (90–98) 92

*Calculated sensitivity based on the accumulated discrepancy numbers under the dilution fold

Main Article

1These first authors contributed equally to this article.

2These authors contributed equally to this article.

Page created: August 25, 2020
Page updated: September 17, 2020
Page reviewed: September 17, 2020
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