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Volume 26, Number 10—October 2020
Dispatch

Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCR

So Yeon Kim1, Jaehyeon Lee1, Heungsup Sung, Hyukmin Lee, Myung Guk Han, Cheon Kwon Yoo, Sang Won Lee2, and Ki Ho Hong2Comments to Author 
Author affiliations: National Medical Center, Seoul, South Korea (S.Y. Kim); Jeonbuk National University Medical School and Hospital, Jeonju, South Korea (J. Lee); Asan Medical Center and University of Ulsan College of Medicine, Seoul (H. Sung); Yonsei University College of Medicine, Seoul (H. Lee); Korea Centers for Disease Control and Prevention, Cheongju (M.G. Han, S.W. Lee, C.K. Yoo); Seoul Medical Center, Seoul (K.H. Hong)

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Abstract

To validate the specimen-pooling strategy for real-time reverse transcription PCR detection of severe acute respiratory syndrome coronavirus 2, we generated different pools including positive specimens, reflecting the distribution of cycle threshold values at initial diagnosis. Cumulative sensitivities of tested pool sizes suggest pooling of <6 specimens for surveillance by this method.

After the first report of the coronavirus disease (COVID-19) outbreak in Wuhan, China (1), the World Health Organization announced pandemic status on March 11, 2020 (2). Real-time reverse transcription PCR (rRT-PCR) detection of the causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a confirmatory diagnostic tool for COVID-19 (3).

A mass screening test for COVID-19 is urgently needed in South Korea because of the increasing number of confirmed cases in long-term care hospitals and public facilities, as well as imported cases. Testing specimens pooled before RNA extraction and subsequently retesting single specimens from positive pools is an efficient strategy for rapid mass screening as well as for increasing testing capacity and conserving resources.

Testing pooled specimens is a well-known method and has been used in blood banks worldwide to screen for infectious disease; however, only a few studies have evaluated specimen pooling for SARS-CoV-2 (4,5; R. Hanel et al., unpub. data. https://arxiv.org/abs/2003.09944v1; M.J. Farfan et al., unpub. data, https://doi.org/10.1101/2020.04.15.20067199). Therefore, we evaluated the pooling strategy for SARS-CoV-2 testing using clinical specimens from 3 hospitals in South Korea: Seoul Medical Center and National Medical Center, both in Seoul, and Jeonbuk National University Hospital in Jeonju. The Institutional Review Boards of the hospitals approved this study. Written consent from participants was waived.

The Study

Pooled upper respiratory specimens were prepared from 50 individual SARS-CoV-2–positive specimens and 300 individual SARS-CoV-2–negative specimens. Either a single nasopharyngeal swab (NPS) or a nasopharyngeal and an oropharyngeal swab (NPS/OPS) were collected in an eNAT tube (Copan Italy, https://www.copangroup.com). Laboratory diagnosis of SARS-CoV-2 infection was performed with all specimens using the following rRT-PCR kits targeting the E and RdRp genes: STANDARD M nCoV Real-time Detection (SD Biosensor, https://sdbiosensor.com) or PowerCheck 2019-nCoV Real-Time Detection (Kogene Biotech, https://www.kogene.co.kr).

Figure 1

Thumbnail of Distribution of RdRp gene threshold cycle (Ct) values for specimens from 4,364 confirmed patients in South Korea at their initial diagnosis of coronavirus disease (COVID-19) and the specimens selected by stratified sampling. This figure shows the first RdRp gene Ct values of patients receiving a COVID-19 diagnosis (bars). We selected positive samples with the stratified sampling method based on that distribution (line). Cumulative numbers of selected specimens per stratum are shown.

Figure 1. Distribution of RdRp gene (Ct) values for specimens from 4,364 confirmed patients in South Korea at their initial diagnosis of coronavirus disease (COVID-19) and the specimens selected by stratified sampling. This...

For the SARS-CoV-2–positive pooled specimens, we selected 50 individual SARS-CoV-2–positive specimens on the basis of the observed population distribution of cycle threshold (Ct) values of r RT-PCR for patients confirmed positive during January 20–March 2, 2020 (Figure 1). We grouped the Ct values into 8 strata, decided the sampling number adequate for each stratum, and selected a total of 50 specimens for 8 strata (Figure 1). We pooled the selected individual SARS-CoV-2–positive specimens with different numbers of SARS-CoV-2–negative specimens to generate 50 sets of pooled specimens in duplicate; the pool sizes of each set were 2, 4, 6, 8, 10, and 16. We prepared a total of 600 pooled specimens. To evaluate clinical specificity in SARS-CoV-2–negative pooled specimens, we randomly combined 16 specimens from 300 negative specimens and generated 60 negative pooled specimens (Appendix).

The following 3 automated RNA extraction systems were used: MagNa Pure 96 (Roche Diagnostics, https://www.roche.com), Real-prep (BioSewoom, www.biosewoom.com), and eMAG (bioMérieux, https://www.biomerieux.com). We followed the extraction protocol provided by each manufacturer with an input volume of 200 μL and elution volume of 50 μL.

We performed rRT-PCR using PowerCheck 2019-nCoV for all pooled specimens. The interpretation guideline by the manufacturer for SARS-CoV-2 positivity was a Ct cutoff of <35 for a single specimen; however, we assessed every amplified curve throughout 40 total PCR cycles. For either the E or RdRp gene, when we observed any amplified curve before the end of the 40 amplification cycles, we interpreted the result as positive for the pooled specimens. When we observed no amplification curves for both genes, we interpreted the result as negative.

We performed all statistical analyses with MedCalc version 19.2.1 (MedCalc Software Ltd, https://www.medcalc.org). The distribution of Ct values in individual specimens (Figure 1) showed negative skewness. In total, 61% of confirmed cases had Ct >30, which was near the cutoff value. We selected positive samples for pooling according to this distribution pattern.

Figure 2

Thumbnail of Mean Ct values of RdRp genes of 50 specimens from coronavirus disease patients in South Korea by pool size. The trend line shows logarithmic regression. Ct, cycle threshold.

Figure 2. Mean Ct values of RdRp genes of 50 specimens from coronavirus disease patients in South Korea by pool size. The trend line shows logarithmic regression. Ct, cycle threshold.

The pooled positive specimens had 100% sensitivity in pool sizes 2, 4, and 6 and 97%–99% sensitivity in pool sizes 8, 10, and 16 (Table). To ensure a conservative estimation of sensitivity, we calculated the cumulative sensitivities on the assumption that the false-negative results that occurred in smaller pool sizes could also occur in larger pool sizes. Therefore, every negative result that occurred in smaller pool sizes was included in the calculation of cumulative sensitivities in larger pool sizes. The cumulative sensitivities of pool size 6 was 100%, of 8, 97%, of 10, 96%, and of 16, 92%. The clinical specificity of pool size 16 was 97% (58/60, 95% CI 87%−99%). The mean Ct values increased for both the E and RdRp genes as the pool size increased (Figure 2; Appendix Figure).

Conclusions

We evaluated the clinical sensitivity and specificity of SARS-CoV-2 rRT-PCR using pooled upper respiratory specimens from confirmed cases. Because pooled specimens are expected to be used as a screening tool, the clinical sensitivity of pooled specimens at a given pool size is especially important.

A limitation of previous studies is that the Ct values of positive specimens from patients at the time of diagnosis were not considered in the study design. The Ct values of specimens in previous studies were relatively low (6). Because specimens with high Ct values, meaning low virus titers, are expected to be vulnerable to pooling, the distribution of Ct values in the actual population should be reflected when determining the pool size. We analyzed the actual distribution of Ct values from 4,364 initially confirmed cases, and the distribution showed skewness with regard to the PCR cutoff value.

Yelin et al. (4) suggested that the pool size using RNA extracts could be <64; however, we do not recommend increasing the pool size to 64, corresponding to a theoretical increase in Ct values of 6, given the associated loss in sensitivity; doing so may cause false negative results.

The pooling strategy showed efficiency when the positive rates in the population were low (7). We showed clinical sensitivities and cumulative sensitivities of the pooled specimens that were sampled after stratification by data, including low viral titers. On the basis of our results, we recommend pooling <6 specimens in clinical practice. Pooling >6 specimens might cause false-negative results, considering the observed abundance of specimens with high Ct values in the population.

This study has some limitations. First, the analytical performance of the PCR kit used has not been evaluated fully because it is one of the earliest available commercial PCR kits that received the Emergency Use Authorization in Korea. Second, the positive cutoff in the kit was a Ct value <35 within 40 amplification cycles. Therefore, this study did not include individual specimens with a Ct value >35, which is interpreted as an inconclusive result by this kit. Third, we did not evaluate cost-effectiveness on the basis of the hypothesized prevalence. Last, we did not evaluate the effect of specimen volume in the pools; increasing the input volume from each specimen may improve the sensitivity of the pooling test.

Our protocol will be helpful for screening persons in groups at high risk for COVID-19 infection quickly and quarantining those confirmed positive, even in situations with limited time and test resources. Epidemiologic factors should be considered when choosing an adequate pooling number. Symptomatic case-patients should be tested individually without pooling to enable effective and timely action. We have included practical guidelines for specimen-pooling procedures in the Appendix.

Dr. Kim is a medical doctor and director of the Department of Laboratory Medicine, National Medical Center, Seoul, South Korea. Her research interests include molecular diagnostics and infectious diseases. Dr. Lee is a medical doctor and assistant professor in the Department of Laboratory Medicine, Jeonbuk National University Medical School and Hospital, Jeonju, South Korea. His research interests include clinical microbiology and infectious disease.

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Acknowledgments

We thank Mallikarjun Handigund for his contribution in generating the data for this study, and Joongyub Lee for his contribution to the statistical analysis. We thank all laboratory technologists in the Department of Molecular Diagnostics at National Medical Center, Jeonbuk National University Hospital, and Seoul Medical Center for their contributions in generating the data for this study.

Author contributions: S.W.L. and K.H.H. take responsibility for the integrity of the data and the accuracy of the data analysis. S.Y.K. and J.L. contributed equally to this study.

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References

  1. Zhu  N, Zhang  D, Wang  W, Li  X, Yang  B, Song  J, et al.; China Novel Coronavirus Investigating and Research Team. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med. 2020;382:72733. DOIPubMed
  2. World Health Organization. WHO Director-General’s opening remarks at the media briefing on COVID-19—11 March 2020. Geneva: The Organization; 2020 [cited 2020 Aug 19]. https://www.who.int/dg/speeches/detail/who-director-general-s-opening-remarks-at-the-media-briefing-on-covid-19---11-march-2020
  3. Hong  KH, Lee  SW, Kim  TS, Huh  HJ, Lee  J, Kim  SY, et al. Guidelines for laboratory diagnosis of coronavirus disease 2019 (COVID-19) in Korea. Ann Lab Med. 2020;40:35160. DOIPubMed
  4. Yelin  I, Aharony  N, Shaer Tamar  E, Argoetti  A, Messer  E, Berenbaum  D, et al. Evaluation of COVID-19 RT-qPCR test in multi-sample pools. Clin Infect Dis. 2020;ciaa531. DOIPubMed
  5. Hogan  CA, Sahoo  MK, Pinsky  BA. Sample pooling as a strategy to detect community transmission of SARS-CoV-2. JAMA. 2020;323:19679. DOIPubMed
  6. Lohse  S, Pfuhl  T, Berkó-Göttel  B, Rissland  J, Geißler  T, Gärtner  B, et al. Pooling of samples for testing for SARS-CoV-2 in asymptomatic people. Lancet Infect Dis. 2020;S1473-3099(20)30362-5. DOIPubMed
  7. European Centre for Disease Prevention and Control. Methodology for estimating point prevalence of SARSCoV-2 infection by pooled RT-PCR testing. Stockholm: The Centre; 2020 [cited 2020 Aug 19]. https://www.ecdc.europa.eu/en/publications-data/methodology-estimating-point-prevalence-sars-cov-2-infection-pooled-rt-pcr

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Figures
Table

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Cite This Article

DOI: 10.3201/eid2610.201955

Original Publication Date: August 25, 2020

1These first authors contributed equally to this article.

2These authors contributed equally to this article.

Table of Contents – Volume 26, Number 10—October 2020

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Ki Ho Hong, Department of Laboratory Medicine, Seoul Medical Center, 156 Sinnae-ro, Jungnang-gu, Seoul, 02053, South Korea

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Page created: August 25, 2020
Page updated: September 17, 2020
Page reviewed: September 17, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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