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Volume 26, Number 12—December 2020
Dispatch

Circulation of 2 Barmah Forest Virus Lineages in Military Training Areas, Australia

Wenjun LiuComments to Author , Joanne R. Kizu, David R. Matley, Richard Grant, Fiona J. McCallum, Christopher G. Moller, Tracy L. Carthew, Jun Hang, Ania J. Gubala, and John G. Aaskov
Author affiliations: Australian Defence Force Malaria and Infectious Disease Institute, Enoggera, Queensland, Australia (W. Liu, J.R. Kizu, R. Grant, F.J. McCallum, C.G. Moller, T.L. Carthew); Enoggera Health Centre, Brisbane, Queensland, Australia (D.R. Matley); Walter Reed Army Institute of Research, Silver Spring, Maryland, USA (J. Hang); Australian Defence Science and Technology Group, Fishermans Bend, Victoria, Australia (A.J. Gubala); Institute of Health and Biomedical Innovation, Brisbane (J.G. Aaskov)

Main Article

Figure 2

Phylogenies based on 17 complete BFV E2 sequences (1,263 bp) (A) and twelve 3′ untranslated region sequences (B) classify Barmah Forest virus isolates into 3 distinct lineages. We used Bayesian phylogenetic analysis method in Geneious version 11.2 (https://www.geneious.com) to analyze the aligned E2 and 3′ untranslated region sequences, applying the Hasegawa-Kishino-Yano plus gamma substitution model with a gamma molecular clock model of uniform branch lengths, a chain length of 1 million, and a 10% burn-in length. The naming convention of the strains is name of host/strain/year of isolation/GenBank accession number. Scale bar indicates the length of the branches of each tree. H, humans; M, mosquitoes; BM, biting midges.

Figure 2. Phylogenies based on 17 complete BFV E2 sequences (1,263 bp) (A) and twelve 3′ untranslated region sequences (B) classify Barmah Forest virus isolates into 3 distinct lineages. We used Bayesian phylogenetic analysis method in Geneious version 11.2 (https://www.geneious.com) to analyze the aligned E2 and 3′ untranslated region sequences, applying the Hasegawa-Kishino-Yano plus gamma substitution model with a gamma molecular clock model of uniform branch lengths, a chain length of 1 million, and a 10% burn-in length. The naming convention of the strains is name of host/strain/year of isolation/GenBank accession number. Scale bar indicates the length of the branches of each tree. H, humans; M, mosquitoes; BM, biting midges.

Main Article

Page created: October 20, 2020
Page updated: November 19, 2020
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