Figure 2. Phylogenies based on 17 complete BFV E2 sequences (1,263 bp) (A) and twelve 3′ untranslated region sequences (B) classify Barmah Forest virus isolates into 3 distinct lineages. We used Bayesian phylogenetic analysis method in Geneious version 11.2 (https://www.geneious.com) to analyze the aligned E2 and 3′ untranslated region sequences, applying the Hasegawa-Kishino-Yano plus gamma substitution model with a gamma molecular clock model of uniform branch lengths, a chain length of 1 million, and a 10% burn-in length. The naming convention of the strains is name of host/strain/year of isolation/GenBank accession number. Scale bar indicates the length of the branches of each tree. H, humans; M, mosquitoes; BM, biting midges.