Volume 26, Number 4—April 2020
Research
Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium
, Kevin Vanneste, Bert Bogaerts, Sigrid C.J. De Keersmaecker, Nancy H. Roosens, Freddy Haesebrouck, Lieze De Cremer, Piet Deprez, Bart Pardon, and Filip Boyen
Table 3
Overview of clustered AMR genes in bovine Gallibacterium anatis isolates, Belgium, 2017–2018*
| Isolate(s) | Clustered AMR genes† | Linked transposases or IME‡ | Predicted contig origin§ |
|---|---|---|---|
| GB4, GB9, GB11 | aac6-aph2, aph3-III, ermB | Putative IME | Chromosome (0.968–0.971) |
| GB7 | aadA1, aadB, catA1 | TnAs3 transposase A. salmonicida | Chromosome (0.988) |
| GB2 | aadA1, aadB, catA1, ermB, tetM | TnAs3 transposase A. salmonicida | Chromosome (0.965) |
| GB3 | aadA1, aadB, sul1, tetM | TnAs3 transposase A. salmonicida | Chromosome (0.98) |
| GB5 | aadA1, catA1, dfrA1, ermB, tetM | TnAs3 transposase A. salmonicida | Chromosome (0.979) |
| GB4, GB9, GB11 | aadA1, catA1, dfrA1, tetM | TnAs3 transposase A. salmonicida | Chromosome (0.99) |
| GB10 | aadA1, catA1, ermB, tetM | TnAs3 transposase A. salmonicida | Chromosome (0.977) |
| GB6 | aadA1, dfrA1, ermB, floR, sul1, tetM | TnAs3 transposase A. salmonicida | Chromosome (0.986) |
| GB8 | aadA23, catA1, dfrA1, ermB, tetM | TnAs3 transposase A. salmonicida | Chromosome (0.957) |
| GB8 | aadB, aphA1 | Truncated IS6 family transposase | Chromosome (0.848) |
| GB5 | aadB, floR | IS6 family transposase | Plasmid (0.694); B. trehalosi pCCK13698 (75%–99%) |
| GB7 | aphA1, catA3, strA, strB, sul2 | ISapl1 transposase A. pleuropneumoniae | Chromosome (0.988) |
| GB2 | aphA1, catA3, strA, strB, sul2 | Truncated IS4 family transposase | Plasmid (0.749); uncultured Eubacterium pIE1130 (84%, 99%) |
| GB10 | aphA1, floR, strA, tetB | ISVsa3 transposase V. salmonicida | Plasmid (0.807); B. trehalosi USDA-ARS-USMARC-192 (68%, 99%) |
| GB3, GB6 | aphA1, sul2 | Truncated ISVsa3 transposase V. salmonicida | Plasmid (0.898); P. multocida USDA-ARS-USMARC-60675 (83%, 99%) |
| GB4, GB9, GB11 | blaTEM-2, strA, sul2, tetB | Tn3 transposase Salmonella | Plasmid (0.864–0.895); S. sonnei p866 (83%, 99%) |
| GB3, GB6 | blaTEM-2, tetB | Tn3 transposase Salmonella | Chromosome (0.976) |
| GB7 | blaTEM-2, tetB | Tn3 transposase Salmonella | Plasmid (0.708); Salmonella Heidelberg pN13–01290_23 (100%, 99%) |
| GB8 | catA3, mphE, msrE, strA, sul2, tetB | Truncated ISVsa5 transposase V. salmonicida | Plasmid (0.738); P. multocida 14424 (71%, 99%) |
| GB5 | strA, tetB | Not detected | Chromosome (0.526) |
*Includes predicted transposases in close proximity of the resistance gene clusters (or predicted IME containing the AMR gene cluster) and the predicated contig origin. AMR, antimicrobial resistance; IME, integrative mobilizable elements.
†AMR genes present on the same contig (genes are listed in alphabetical order).
‡Determined by using ISfinder for transposases and ICEberg for IME.
§Determined by using mlplasmids. Values in parentheses indicate (range of) posterior probability of belonging to either a plasmid or chromosome. For predicted plasmids, the best hit in the National Center for Biotechnology Information nucleotide database is also listed, with its corresponding query coverage and percentage identity, respectively, in parentheses.