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Volume 26, Number 6—June 2020
Research

Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States

Jennifer Harcourt1, Azaibi Tamin1, Xiaoyan Lu, Shifaq Kamili, Senthil K. Sakthivel, Janna Murray, Krista Queen, Ying Tao, Clinton R. Paden, Jing Zhang, Yan Li, Anna Uehara, Haibin Wang, Cynthia Goldsmith, Hannah A. Bullock, Lijuan Wang, Brett Whitaker, Brian Lynch, Rashi Gautam, Craig Schindewolf, Kumari G. Lokugamage, Dionna Scharton, Jessica A. Plante, Divya Mirchandani, Steven G. Widen, Krishna Narayanan, Shinji Makino, Thomas G. Ksiazek, Kenneth S. Plante, Scott C. Weaver, Stephen Lindstrom, Suxiang Tong, Vineet D. Menachery2, and Natalie J. Thornburg2Comments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Harcourt, A. Tamin, X. Lu, K. Queen, Y. Tao, C.R. Paden, Y. Li, C. Goldsmith, B. Whitaker, R. Gautam, S. Lindstrom, S. Tong, N.J. Thornburg); Eagle Medical Services, Atlanta (S. Kamili, S.K. Sakthivel, J. Murray, B. Lynch); IHRC, Atlanta (J. Zhang, H. Wang); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (A. Uehara); Synergy America, Inc., Atlanta (H.A. Bullock, L. Wang); University of Texas Medical Branch, Galveston, Texas, USA (C. Schindewolf, K.G. Lokugamage, D. Mirchandani, S. Widen, K. Narayanan, S. Makino, T.G. Ksiazek, S.C. Weaver, V.D. Menachery); World Reference Center for Emerging Viruses and Arboviruses, Galveston (D. Scharton, J.A. Plante, T.G. Ksiazek, K.S. Plante, S.C. Weaver, V.D. Menachery)

Main Article

Figure 4

Multistep growth curve for severe acute respiratory syndrome coronavirus 2 from patient with 2019 novel coronavirus disease, United States, 2020. Vero CCL81 (black) and HUH7.0 cells (green) were infected at a multiplicity of infection of 0.1, and cells (solid line) and supernatants (dashed line) were harvested and assayed for viral replication by using TCID50. Circles, Vero CCL81 cells; squares, Vero CCL81 supernatants; triangles, HUH7.0 cells; inverted triangles, HUH7.0 supernatants. Error bars

Figure 4. Multistep growth curve for severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States, 2020. Vero CCL81 (black) and HUH7.0 cells (green) were infected at a multiplicity of infection of 0.1, and cells (solid line) and supernatants (dashed line) were harvested and assayed for viral replication by using TCID50. Circles, Vero CCL81 cells; squares, Vero CCL81 supernatants; triangles, HUH7.0 cells; inverted triangles, HUH7.0 supernatants. Error bars indicate SEM. TCID50, 50% tissue culture infectious dose.

Main Article

1These authors contributed equally to this article.

2These senior authors contributed equally to this article.

Page created: May 18, 2020
Page updated: May 18, 2020
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