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Volume 26, Number 8—August 2020
Dispatch

Mother-to-Child Transmission of Andes Virus through Breast Milk, Chile1

Marcela FerrésComments to Author , Constanza Martínez-Valdebenito, Jenniffer Angulo, Carolina Henríquez, Jorge Vera-Otárola, María José Vergara, Javier Pérez, Jorge Fernández, Viviana Sotomayor, María Francisca Valdés, Diego González-Candia, Nicole D. Tischler, Cecilia Vial, Pablo Vial, Gregory Mertz, and Nicole Le CorreComments to Author 
Author affiliations: Pontificia Universidad Católica de Chile, Santiago, Chile (M. Ferrés, C. Martinez-Valdebenito, J. Angulo, C. Henriquez, J. Vera-Otarola, M.J. Vergara, J. Perez, N. Le Corre); Instituto de Salud Pública de Chile, Santiago (J. Fernandez); Ministerio de Salud de Chile, Santiago (V. Sotomayor, M.F. Valdes); Fundación Ciencia & Vida, Santiago (D. Gonzalez-Candia, N. Tischler); Universidad San Sebastián, Santiago (N. Tischler); Universidad del Desarrollo, Santiago (C. Vial, P. Vial); University of New Mexico, Albuquerque, New Mexico, USA (G. Mertz)

Main Article

Figure 2

Detection of ANDV N and Gc proteins from enucleated cells from breast milk from a mother in Chile. A) Detection of N protein and the cytoplasmic marker eIF3. B) Detection of N protein with 2 different primary antibodies. C) Detection of N and Gc proteins. Huh-7 cells were mock-infected (mock column), ANDV infected (ANDV column), or incubated with a pellet from breast milk (breast milk column). Coverslips were incubated with mouse and rabbit antibodies. Scale bars indicate 20 µm. Complete methods

Figure 2. Detection of ANDV N and Gc proteins from enucleated cells from breast milk from a mother in Chile. A) Detection of N protein and the cytoplasmic marker eIF3. B) Detection of N protein with 2 different primary antibodies. C) Detection of N and Gc proteins. Huh-7 cells were mock-infected (mock column), ANDV infected (ANDV column), or incubated with a pellet from breast milk (breast milk column). Coverslips were incubated with mouse and rabbit antibodies. Scale bars indicate 20 µm. Complete methods are described in the Appendix. ANDV, Andes virus; Gc, glycoprotein; N, nucleoprotein.

Main Article

1Preliminary results from this study were presented at 11th International Conference on Hantaviruses, September 1-4, 2019, Leuven, Belgium.

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