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Volume 26, Number 8—August 2020
Research Letter

SARS-CoV-2 Transmission from Presymptomatic Meeting Attendee, Germany

DirkJan HijnenComments to Author , Angelo Valerio Marzano, Kilian Eyerich, Corine GeurtsvanKessel, Ana Maria Giménez-Arnau, Pascal Joly, Christian Vestergaard, Michael Sticherling, and Enno Schmidt
Author affiliations: Erasmus MC University Medical Center Rotterdam, Rotterdam, the Netherlands (D. Hijnen, C. GeurtsvanKessel); Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy (A.V. Marzano); Università degli Studi di Milano, Milan (A.V. Marzano); Karolinska Institutet, Solna, Sweden (K. Eyerich); Universitat Autònoma de Barcelona, Barcelona, Spain (A.M. Giménez-Arnau); INSERM U—Rouen University Hospital, Rouen, France (P. Joly); Aarhus University Hospital, Aarhus, Denmark (C. Vestergaard); University Hospitals Erlangen, Erlangen, Germany (M. Sticherling); Lübeck Institute of Experimental Dermatology, Lübeck, Germany (E. Schmidt); University of Lübeck, Lübeck (E. Schmidt)

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Abstract

During a meeting in Munich, Germany, a presymptomatic attendee with severe acute respiratory syndrome coronavirus 2 infected at least 11 of 13 other participants. Although 5 participants had no or mild symptoms, 6 had typical coronavirus disease, without dyspnea. Our findings suggest hand shaking and face-to-face contact as possible modes of transmission.

We describe efficient spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) resulting from contact with a presymptomatic infected person during a scientific advisory board meeting held February 20–21, 2020, in Munich, Germany; the country had <20 diagnosed coronavirus disease (COVID-19) cases at the time. Eight dermatologists from 7 countries and 6 scientists from the same company attended the meeting at a hotel in central Munich. The meeting was held in a room (≈70 m2) with conventional radiators; a U-shaped setup of tables were separated by a central aisle >1 m wide. During the meeting, refreshments were served buffet style in the same room 4 times. In addition to 9.5 hours of discussions, the participants had dinner on February 20 in a nearby restaurant. Additional direct contacts between participants were handshakes during welcome and farewell with few short hugs without kisses. None of the participants, including the index patient (participant [Pt] 1), showed any signs of infection (e.g., coughing, sneezing, respiratory symptoms, shivering, fever) before or during the meeting. No one wore a mask during the meeting. After the meeting, the index patient (Pt 1) shared a taxi with Pt 2, 4, and 9 for ≈45 min.

After returning home the evening of February 21, Pt 1 sought care for fever. Reverse transcription PCR was performed on throat and nasal swab specimens, and SARS-CoV-2 RNA was detected by established methods (1). The patient was admitted to the hospital for supportive care, although he had only moderate symptoms (Table).

National authorities contacted most meeting participants on February 26 (Pt 7, 11–13) and 27 (Pt 2–6, 8–12); Pt 14 was contacted by a coworker. Twelve participants, including Pt 1, were tested for SARS-CoV-2 by PCR; 2 were not, Pt 9 because he showed no signs of infection and Pt 6 because testing was not available at his location (New York, NY, USA) at the time. Pt 6 later underwent ELISA testing, which showed IgA and IgG against the recombinant S1 domain of structural protein of SARS-CoV-2 (Euroimmun, https://www.euroimmun.com) (2). Excluding the index patient, in 10/11 tested participants, SARS-CoV-2 RNA was detected. In 1 participant, Pt 11, the PCR result for SARS-CoV-2 RNA was negative (Table). Thus, the index patient infected >11 (85%) of the 13 other participants.

All participants were isolated either in a hospital or at home with or without their families, regardless of the outcome of the first PCR test. These measures resulted in the subsequent infection of 14 additional persons (Table). Of the 12 infected participants, 2 (17%) had no symptoms, 3 (25%) experienced mild influenza-like symptoms, and 7 (58%) experienced a considerable reduction of their health, without dyspnea, classified as moderate COVID-19 (Table). None of the participants had a relevant medical history.

The index patient (Pt 1) was most likely infected by an outpatient he had examined in Milan, Italy, on February 18. The index patient reported that he had experienced no symptoms when attending the meeting. Probable transmission of SARS-CoV-2 from presymptomatic persons has been reported (3,4), with viral load levels in the nose similar to those of symptomatic patients (5). In contrast to severe acute respiratory syndrome coronavirus and influenza virus, the infectiousness of SARS-CoV-2 peaks on or before symptom onset (6).

The exact mode of transmission during the meeting remains elusive. At least 4 routes have been suggested: droplets during face-to-face contacts, aerosolized droplets (<5 µm) via air flow, fomites, and hand shaking (4,79). We identified face-to-face contacts lasting >5 min with the index patient and the 11 infected participants during 2 lunches (30 min each), 2 coffee breaks (15 min each), and the social dinner (sitting close to Pt 2, 4, 5, and 11). We also tracked Pt 1 sitting next to Pt 3 and Pt 6 during the meeting, and a 45-min taxi ride after the meeting (with Pt 2, 4, and 9) (Table). The index patient sat ≈2.60 m away from the closest participant opposite to him and had an average talk time during the meeting. Virus aerosolization in the relatively small room that was heated by conventional radiators appears to be possible in light of the duration of the meeting. Transmission via fomites appears to be less likely because few objects (bottles, coffee pots, forks) were shared by all participants during the breaks. Telephone communication with hotel management on April 20 revealed that none of the involved hotel staff were tested for SARS-CoV-2 and no staff member reported symptoms consistent with COVID-19.

Our findings indicate that hand shaking, aerosolization, and face-to-face contact may be relevant modes of transmission in this COVID-19 outbreak. Limitations include the lack of environmental samples and data about room ventilation and airflow patterns, as well as missing information about the infection status of Pt 9 and the inability to determine the actual impact of SARS-CoV-2 transmission from handshakes, droplets, and aerosolization.

Dr. Hijnen is a dermatologist, instructor, and researcher at Erasmus MC University Medical Center Rotterdam. His primary research interests are T-cell immunology and precision medicine in atopic dermatitis.

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Acknowledgment

We thank the company and the participating scientists for their contributions and support.

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References

  1. World Health Organization. Country & technical guidance—coronavirus disease (COVID-19) [cited 2020 May 7]. https://www.who.int/emergencies/diseases/novel-coronavirus-2019/technical-guidance
  2. Okba  NMA, Müller  MA, Li  W, Wang  C, GeurtsvanKessel  CH, Corman  VM, et al. Severe acute respiratory syndrome coronavirus 2−specific antibody responses in coronavirus disease 2019 patients. Emerg Infect Dis. 2020;26. DOIPubMed
  3. Li  P, Fu  JB, Li  KF, Chen  Y, Wang  HL, Liu  LJ, et al. Transmission of COVID-19 in the terminal stage of incubation period: a familial cluster. Int J Infect Dis. 2020;S1201-9712(20)30146-6; Epub ahead of print. DOIPubMed
  4. Tong  ZD, Tang  A, Li  KF, Li  P, Wang  HL, Yi  JP, et al. Potential presymptomatic transmission of SARS-CoV-2, Zhejiang Province, China, 2020. Emerg Infect Dis. 2020;26:10524. DOIPubMed
  5. Zou  L, Ruan  F, Huang  M, Liang  L, Huang  H, Hong  Z, et al. SARS-CoV-2 viral load in upper respiratory specimens of infected patients. N Engl J Med. 2020;382:11779. DOIPubMed
  6. He  X, Lau  EHY, Wu  P, Deng  X, Wang  J, Hao  X, et al. Temporal dynamics in viral shedding and transmissibility of COVID-19. Nat Med. 2020. DOIPubMed
  7. Cai  J, Sun  W, Huang  J, Gamber  M, Wu  J, He  G. Indirect virus transmission in cluster of COVID-19 cases, Wenzhou, China, 2020. Emerg Infect Dis. 2020;26. DOIPubMed
  8. Lu  J, Gu  J, Li  K, Xu  C, Su  W, Lai  Z, et al. COVID-19 outbreak associated with air conditioning in restaurant, Guangzhou, China, 2020. Emerg Infect Dis. 2020;26. DOIPubMed
  9. van Doremalen  N, Bushmaker  T, Morris  DH, Holbrook  MG, Gamble  A, Williamson  BN, et al. Aerosol and surface stability of SARS-CoV-2 as compared with SARS-CoV-1. N Engl J Med. 2020;382:15647. DOIPubMed

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Cite This Article

DOI: 10.3201/eid2608.201235

Original Publication Date: May 11, 2020

Table of Contents – Volume 26, Number 8—August 2020

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DirkJan Hijnen, Erasmus MC University Medical Center Rotterdam, Department of Dermatology, Rotterdam, The Netherlands

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Page created: May 11, 2020
Page updated: July 19, 2020
Page reviewed: July 19, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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