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Volume 27, Number 1—January 2021
Research Letter

Waning Antibody Responses in Asymptomatic and Symptomatic SARS-CoV-2 Infection

Pyoeng Gyun Choe1, Chang Kyung Kang1, Hyeon Jeong Suh, Jongtak Jung, Kyoung-Ho Song, Ji Hwan Bang, Eu Suk Kim, Hong Bin Kim, Sang Won Park, Nam Joong Kim, Wan Beom ParkComments to Author , and Myoung-don OhComments to Author 
Author affiliation: Seoul National University College of Medicine, Seoul, South Korea

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Abstract

We investigated the kinetics of severe acute respiratory syndrome coronavirus 2 neutralizing antibodies in 7 asymptomatic persons and 11 patients with pneumonia. The geometric mean titer of neutralizing antibodies declined from 219.4 at 2 months to 143.7 at 5 months after infection, indicating a waning antibody response.

Neutralizing antibodies develop in asymptomatic persons with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection; however, the initial immune response is not as strong as in patients with more severe disease (1,2). We investigated the kinetics of SARS-CoV-2 neutralizing antibodies during the 5 months after infection in asymptomatic persons and patients with pneumonia caused by SARS-CoV-2.

We studied 7 persons infected with SARS-CoV-2 who were isolated in a community treatment center operated by Seoul National University (SNU) Hospital in Daegu, South Korea (3). Comprehensive monitoring confirmed that these 7 patients were asymptomatic (4). We also evaluated 11 SARS-CoV-2–positive patients with pneumonia at the Biocontainment Unit in the SNU Hospital and SNU Bundang Hospital. We classified each case of pneumonia as subtle (i.e., infiltrations observed only on computed tomography) or apparent (i.e., infiltrations observed on plain chest radiograph) (Appendix). All patients provided informed consent.

We evaluated the antibody responses at 2 and 5 months after infection, as reported (1). We semiquantitatively measured IgG against SARS-CoV-2 using ELISA (Euroimmun, https://www.euroimmun.com) with the recombinant S1 domain of the SARS-CoV-2 spike protein as the antigen. We interpreted the optical density ratio (sample/calibrator) as negative (<0.8), borderline (>0.8 to <1.1), or positive (>1.1), according to the manufacturer’s recommendations. We also conducted neutralization assays as previously described (5) using BetaCoV/Korea/SNU01/2020 virus (6) and 2-fold serially diluted plasma samples (2–4,096-fold). We recorded the highest dilution of plasma that showed inhibition activity of SARS-CoV-2 as the neutralizing antibody titer. We considered a >4-fold reduction in antibody titer to be a waning response. The Institutional Review Boards of Seoul National University Hospital approved the study (IRB no. H-2004-158-1118).

Two months after infection, 11 (100%) patients with pneumonia and 5 (71%) with asymptomatic infection had positive ELISA results. Five months after infection, 5 (100.0%) patients with apparent pneumonia, 5 (83.3%) with subtle pneumonia, and 4 (57.1%) with asymptomatic infection had positive ELISA results. The mean ELISA optical density decreased significantly from 2 to 5 months after infection (4.93 at 2 months vs. 4.09 at 5 months; p = 0.01).

Figure

Waning antibody response against severe acute respiratory virus coronavirus 2, South Korea, 2020. Responses measured by A) ELISA optical density measurements (p = 0.01); B) neutralizing antibody titers (p = 0.03). Each line indicates data from a single patient.

Figure. Waning antibody response against severe acute respiratory virus coronavirus 2, South Korea, 2020. Responses measured by A) ELISA optical density measurements (p = 0.01); B) neutralizing antibody titers (p =...

Two months after infection, all patients had neutralizing antibodies. Antibody titers correlated with disease severity; the geometric mean titer was 105 among symptomatic persons, 161 among patients with subtle pneumonia, and 891 among patients with apparent pneumonia. Five months after infection, all patients still had neutralizing antibodies, but the geometric mean titer decreased significantly (219.4 at 2 months vs. 143.7 at 5 months; p = 0.03). In the linear regression model, the decline was significantly associated with the antibody levels at 2 months as measured by ELISA (r = 0.536, p = 0.02) and the neutralization assay (r = 0.563, p = 0.02) (Appendix Figure). The waning neutralizing antibody response occurred in 2 (40%) of 5 patients with apparent pneumonia and 2 (33%) of 6 with subtle pneumonia, but none of the asymptomatic persons (Figure).

Determining the longevity of humoral immunity to SARS-CoV-2 is essential to predicting herd immunity to coronavirus disease. Among patients with severe acute respiratory syndrome coronavirus, which is closely related to SARS-CoV-2, a total of 90% maintained IgG for 2 years and 50% for 3 years (7). However, humoral immunity to common human coronavirus is short-lived; antibodies against seasonal coronaviruses return to baseline levels by 52 weeks after infection, enabling homologous reinfections (8). A recent study showed that the antibody titers of patients with mild coronavirus disease declined more quickly than did those of patients with severe acute respiratory syndrome (9).

Our findings demonstrate waning humoral immunity in patients with SARS-CoV-2 infection. We documented the decline of neutralizing antibody titers in asymptomatic and symptomatic patients. In this study, the initial neutralizing antibody reaction appeared to correlate with the severity of the disease. However, patients with pneumonia were considerably older than asymptomatic persons, and increasing age is associated with a stronger neutralizing antibody response (10). In this study, neutralizing antibody titer decreased more in symptomatic than asymptomatic patients. Our study reinforces the concern that naturally acquired humoral immunity against SARS-CoV-2 might not be long-lasting.

Dr. Choe is a clinical scientist at Seoul National University Hospital. His research interests focus on preventing healthcare-associated infection and responding to emerging infectious diseases.

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Acknowledgments

We thank Kyung Sook Ahn for administrative support. We thank Areum Jo and Su Jin Choi for technical support.

This project was supported by the research fund of Seoul National University Hospital (grant no. 04-2020-0030). The funding agencies had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.

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References

  1. Choe  PG, Kang  CK, Suh  HJ, Jung  J, Kang  E, Lee  SY, et al. Antibody responses to SARS-CoV-2 at 8 weeks postinfection in asymptomatic patients. Emerg Infect Dis. 2020;26:24847. DOIPubMed
  2. Long  QX, Tang  XJ, Shi  QL, Li  Q, Deng  HJ, Yuan  J, et al. Clinical and immunological assessment of asymptomatic SARS-CoV-2 infections. Nat Med. 2020;26:12004. DOIPubMed
  3. Kang  E, Lee  SY, Jung  H, Kim  MS, Cho  B, Kim  YS. Operating protocols of a community treatment center for isolation of patients with coronavirus disease, South Korea. Emerg Infect Dis. 2020;26:232937. DOIPubMed
  4. Choe  PG, Kang  EK, Lee  SY, Oh  B, Im  D, Lee  HY, et al. Selecting coronavirus disease 2019 patients with negligible risk of progression: early experience from non-hospital isolation facility in Korea. Korean J Intern Med (Korean Assoc Intern Med). 2020;35:76570. DOIPubMed
  5. Shen  C, Wang  Z, Zhao  F, Yang  Y, Li  J, Yuan  J, et al. Treatment of 5 critically ill patients with COVID-19 with convalescent plasma. JAMA. 2020;323:15829. DOIPubMed
  6. Park  WB, Kwon  NJ, Choi  SJ, Kang  CK, Choe  PG, Kim  JY, et al. Virus isolation from the first patient with SARS-CoV-2 in Korea. J Korean Med Sci. 2020;35:e84. DOIPubMed
  7. Wu  LP, Wang  NC, Chang  YH, Tian  XY, Na  DY, Zhang  LY, et al. Duration of antibody responses after severe acute respiratory syndrome. Emerg Infect Dis. 2007;13:15624. DOIPubMed
  8. Callow  KA, Parry  HF, Sergeant  M, Tyrrell  DA. The time course of the immune response to experimental coronavirus infection of man. Epidemiol Infect. 1990;105:43546. DOIPubMed
  9. Ibarrondo  FJ, Fulcher  JA, Goodman-Meza  D, Elliott  J, Hofmann  C, Hausner  MA, et al. Rapid decay of anti-SARS-CoV-2 antibodies in persons with mild Covid-19. N Engl J Med. 2020;383:10857. DOIPubMed
  10. Wang  X, Guo  X, Xin  Q, Pan  Y, Hu  Y, Li  J, et al. Neutralizing antibodies responses to SARS-CoV-2 in COVID-19 inpatients and convalescent patients. Clin Infect Dis. 2020;ciaa721. DOIPubMed

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Figure

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Suggested citation for this article: Choe PG, Kang CK, Suh HJ, Jung J, Song K-H, Bang JH, et al. Waning antibody responses in asymptomatic and symptomatic SARS-CoV-2 infection. Emerg Infect Dis. 2021 Jan [date cited]. https://doi.org/10.3201/eid2701.203515

DOI: 10.3201/eid2701.203515

1These first authors equally contributed to this article.

Table of Contents – Volume 27, Number 1—January 2021

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Wan Beom Park or Myoung-don Oh, Department of Internal Medicine, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-gu, Seoul 03080, South Korea

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Page created: September 28, 2020
Page updated: October 13, 2020 9:21 PM EDT
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