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Volume 27, Number 2—February 2021

Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria Disease

Himanshu Gupta12Comments to Author , Mercedes Rubio1, Antonio Sitoe, Rosauro Varo, Pau Cisteró, Lola Madrid, Inocencia Cuamba, Alfons Jimenez, Xavier Martiáñez-Vendrell, Diana Barrios, Lorena Pantano, Allison Brimacombe, Mariona Bustamante, Quique Bassat3, and Alfredo Mayor3
Author affiliations: ISGlobal, Hospital Clinic—Universitat de Barcelona, Barcelona, Spain (H. Gupta, M. Rubio, R. Varo, P. Cisteró, A. Jimenez, X. Martiáñez-Vendrell, D. Barrios, A. Brimacombe, M. Bustamante, Q. Bassat, A. Mayor); Centro de Investigação em Saúde de Manhiça (CISM), Maputo, Mozambique (A. Sitoe, R. Varo, L. Madrid, I. Cuamba, Q. Bassat, A. Mayor); Spanish Consortium for Research in Epidemiology and Public Health (CIBERESP), Madrid, Spain (A. Jimenez, M. Bustamante, Q. Bassat, A. Mayor); Harvard T.H. Chan School of Public Health Department of Biostatistics, Boston, Massachusetts, USA (L. Pantano); Universitat Pompeu Fabra (UPF), Barcelona (M. Bustamante); I; nstitut Català de Recerca en Estudis Avançats (ICREA), Barcelona (Q. Bassat); Hospital Sant Joan de Déu—University of Barcelona Pediatrics Department, Barcelona (Q. Bassat)

Main Article

Table 3

Association of miRNA levels with severe malaria, symptoms of severity, and Plasmodium falciparum cytoadhesion among children with uncomplicated and severe malaria, Mozambique*

Characteristic miRNA baseMean log2-fold change Adjusted p value
Clinical data
SM, n = 44 vs. 39 UM
All hsa-miR-122-5p 19,929.69 1.67 0.001
SA, n = 17 vs. 39 UM
hsa-miR-4492 17.34 2.81 0.046
hsa-miR-4497 293.66 2.18 0.046
Prostration, n = 33 vs. 39 UM
hsa-miR-122-5p 20,677 1.89 0.001
hsa-miR-6087 5.36 2.39 0.033
hsa-miR-511-5p 126.67 1.36 0.040
Acidosis or respiratory distress, n = 18 vs. 39 UM
hsa-miR-122-5p 13,367.43 2.21 <0.001

Cytoadhesion data
Platelet-mediated agglutination, n = 50 vs. 19 UM
hsa-miR-3158-3p 1,180.96 −2.26 <0.001
hsa-miR-320a 22,005.69 −1.48 0.001
hsa-miR-4492 18.33 2.78 0.002
hsa-miR-1290 1,011.34 −1.38 0.014
hsa-miR-320b 1,191.44 −1.23 0.014
hsa-miR-320c 408.32 −1.29 0.014
hsa-miR-1246 3,907.45 −1.32 0.019
hsa-miR-6741-5p 48.11 −1.81 0.023
hsa-miR-1228-5p 82.73 −1.88 0.023
hsa-miR-3195 16.35 2.21 0.023
hsa-miR-7706 334.86 −1.00 0.023
gC1qR, n = 35 vs. 34 UM
hsa-miR-1-3p 622.35 2.09 0.003

*Positive fold change indicates overexpression in severe malaria and symptoms of severity compared to UM as well as parasites showing cytoadhesion compared to none. Total number of miRNAs in RNA sequencing data was 1,450. p value adjusted for multiple testing by the Benjamini-Hochberg method. baseMean, mean normalized expression of the miRNAs in all the samples; miRNA, microRNA; SA, severe anemia, SM, severe malaria; UM, uncomplicated malaria.

Main Article

1These authors contributed equally to this article.

2Current affiliation: London School of Hygiene and Tropical Medicine, London, UK.

3These senior authors contributed equally to this article.

Page created: December 10, 2020
Page updated: January 23, 2021
Page reviewed: January 23, 2021
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