Volume 27, Number 3—March 2021
Research Letter
Genomic and Pathologic Findings for Prototheca cutis Infection in Cat
Table
Antimicrobial and antifungal MICs and resistance genes identified by whole-genome sequencing of Prototheca cutis isolated from a nasal lesion in a cat*
Class |
MIC, μg/mL |
AMR genes |
---|---|---|
Antimicrobial testing† | ||
β-lactams | Oxa-168, NmcA, OXA-198, IMP-8 | |
Amoxicillin/clavulanate | 8 | |
Ampicillin | >8 | |
Oxacillin | <0.25 | |
Penicillin G | 8 | |
Cefazolin | >4 | |
Cefovecin | >8 | |
Cefpodoxime | >8 | |
Cephalothin | >4 | |
Imipenem | 4 | |
Tetracyclines | tet(31) | |
Doxycycline | >0.5 | |
Minocycline | 1 | |
Tetracycline | >1 | |
Quinolones | None found | |
Enrofloxacin | >4 | |
Marbofloxacin | 2 | |
Pradofloxacin | <0.25 | |
Aminoglycosides | AAC(6')-Ij, AAC(3)-Xa, AAC(3)-VIIIa | |
Amikacin | >32 | |
Gentamicin | 8 | |
Lincosamides: clindamycin | 4 | None found |
Macrolides: erythromycin | >4 | None found |
Rifamycins: rifampin | >2 | None found |
Nitrofurans: nitrofurantoin | >64 | None found |
Phenicols: chloramphenicol | >32 | catB10 |
Sulfonamides: trimethoprim/sulfamethosoxazole | <2 | None found |
Vancomycin |
>16 |
vanA, vanRO |
Antifungal testing‡ | Not investigated | |
Azoles | ||
Fluconazol | >256 | |
Itraconazol | >32 | |
Polyenes: amphotericin B | 0.19 |
*AMR genes were identified by using the Comprehensive Antibiotic Resistance Database (https://card.mcmaster.ca). Interpretation of MICs was not included in this table because clinical breakpoints for Prototheca spp. are not available. AMR, antimicrobial resistance. †Antimicrobial susceptibility testing was performed by using a Trek Sensititre Gram Positive panel (TREK Diagnostic Systems, http://www.trekds.com). ‡Antifungal susceptibility testing was performed by using MIC test strips (Liofilchem, https://www.liofilchem.com).
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1These authors contributed equally to this article.