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Volume 27, Number 3—March 2021
Research Letter

Genomic and Pathologic Findings for Prototheca cutis Infection in Cat

Grazieli Maboni1Comments to Author , Jessica A. Elbert1, Justin M. Stilwell, and Susan Sanchez
Author affiliations: University of Guelph, Guelph, Ontario, Canada (G. Maboni); Athens Veterinary Diagnostic Laboratory, Athens, Georgia, USA (G. Maboni, S. Sanchez); University of Georgia, Athens (J.A. Elbert, J.M. Stilwell)

Main Article


Antimicrobial and antifungal MICs and resistance genes identified by whole-genome sequencing of Prototheca cutis isolated from a nasal lesion in a cat*

MIC, μg/mL
AMR genes
Antimicrobial testing†
β-lactams Oxa-168, NmcA, OXA-198, IMP-8
Amoxicillin/clavulanate 8
Ampicillin >8
Oxacillin <0.25
Penicillin G 8
Cefazolin >4
Cefovecin >8
Cefpodoxime >8
Cephalothin >4
Imipenem 4
Tetracyclines tet(31)
Doxycycline >0.5
Minocycline 1
Tetracycline >1
Quinolones None found
Enrofloxacin >4
Marbofloxacin 2
Pradofloxacin <0.25
Aminoglycosides AAC(6')-Ij, AAC(3)-Xa, AAC(3)-VIIIa
Amikacin >32
Gentamicin 8
Lincosamides: clindamycin 4 None found
Macrolides: erythromycin >4 None found
Rifamycins: rifampin >2 None found
Nitrofurans: nitrofurantoin >64 None found
Phenicols: chloramphenicol >32 catB10
Sulfonamides: trimethoprim/sulfamethosoxazole <2 None found
vanA, vanRO
Antifungal testing‡ Not investigated
Fluconazol >256
Itraconazol >32
Polyenes: amphotericin B 0.19

*AMR genes were identified by using the Comprehensive Antibiotic Resistance Database ( Interpretation of MICs was not included in this table because clinical breakpoints for Prototheca spp. are not available. AMR, antimicrobial resistance.
†Antimicrobial susceptibility testing was performed by using a Trek Sensititre Gram Positive panel (TREK Diagnostic Systems, ‡Antifungal susceptibility testing was performed by using MIC test strips (Liofilchem,

Main Article

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Main Article

1These authors contributed equally to this article.

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Page updated: February 22, 2021
Page reviewed: February 22, 2021
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