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Volume 27, Number 8—August 2021
Dispatch

Natural Human Infections with Plasmodium cynomolgi, P. inui, and 4 other Simian Malaria Parasites, Malaysia

Nan Jiun Yap1, Hanisah Hossain1, Thamayanthi Nada-Raja, Romano Ngui, Azdayanti Muslim, Boon-Peng Hoh, Loke Tim Khaw, Khamisah Abdul Kadir, Paul Cliff Simon Divis, Indra Vythilingam, Balbir SinghComments to Author , and Yvonne Ai-Lian LimComments to Author 
Author affiliations: Universiti Malaya, Kuala Lumpur, Malaysia (N.J. Yap, R. Ngui, A. Muslim, I. Vythilingam, Y.A.-L. Lim); Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia (H. Hossain, T. Nada-Raja, K.A. Kadir, P.C.S. Divis, B. Singh); Universiti Teknologi MARA (Sungai Buloh Campus), Selangor, Malaysia (A. Muslim); UCSI University, Kuala Lumpur, Malaysia (B.-P. Hoh); International Medical University, Kuala Lumpur, Malaysia (L.T. Khaw)

Main Article

Table 2

Comparison between results of nested PCR and sequencing at UM and UNIMAS for identification of Plasmodium malaria species from indigenous community blood samples, Malaysia*

Sample ID Identification at UM
 Identification at UNIMAS
PCR assays based on SSU rRNA genes Phylogenetic analysis of SSU rRNA genes PCR assays based on SSU rRNA genes Phylogenetic analysis of COX1 genes
UM1 P. coatneyi P. coatneyi Negative ND
UM2 P. coatneyi P. coatneyi Negative ND
UM3 P. coatneyi P. coatneyi Negative ND
UM5 P. inui P. inui Negative ND
UM6 P. inui P. inui Positive P. inui–like, P. simiovale
UM7 P. inui P. inui P. inui P. inui-like
UM9 P. cynomolgi P. cynomolgi P. cynomolgi, P. inui P. cynomolgi
UM10 P. cynomolgi Plasmodium spp. P. cynomolgi, P. inui P. cynomolgi
UM11 P. cynomolgi P. cynomolgi P. cynomolgi, P. inui P. cynomolgi
UM12 P. cynomolgi P. cynomolgi P. cynomolgi, P. inui P. cynomolgi
UM14 P. cynomolgi P. cynomolgi P. cynomolgi, P. inui P. cynomolgi
UM15 P. cynomolgi P. cynomolgi P. cynomolgi, P. inui P. cynomolgi
UM16 P. cynomolgi Plasmodium spp. P. cynomolgi, P. inui P. cynomolgi, P. inui–like, P. simiovale
UM17 P. cynomolgi P. cynomolgi P. cynomolgi, P. inui P. cynomolgi
UM18 P. cynomolgi P. cynomolgi P. cynomolgi, P. inui P. cynomolgi

*Negative, negative for Plasmodium DNA and not examined by species-specific nested PCR assays; ND, not done; positive, positive for Plasmodium DNA but negative with species-specific nested PCR assays. SSU, small subunit; UM, Universiti Malaya; UNIMAS, Universiti Malaysia Sarawak.

Main Article

1These authors contributed equally to this article.

Page created: May 14, 2021
Page updated: July 18, 2021
Page reviewed: July 18, 2021
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