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Volume 27, Number 9—September 2021
Research

Development and Clinical Evaluation of a CRISPR-Based Diagnostic for Rapid Group B Streptococcus Screening

Lingxiao Jiang1, Weiqi Zeng1, Wanting Wu1, Yingying Deng, Fusheng He, Wenli Liang, Mingyao Huang, Hong Huang, Yongjun Li, Xiaorui Wang, Hang Su, Shilei PanComments to Author , and Teng Xu1Comments to Author 
Author affiliations: Zhujiang Hospital, Southern Medical University, Guangzhou, China (L. Jiang, Y. Deng, W. Liang, M. Huang); Vision Medicals Center for Medical Research, Shenzhen, China (W. Zeng, W. Wu, H. Huang, Y. Li, X. Wang, H. Su, T. Xu); Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province and College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China (W. Zeng, T. Xu); Zhujiang Hospital, Southern Medical University, Guangzhou (S. Pan)

Main Article

Figure 3

Determination of assay cutoff for CRISPR-based diagnostic for rapid GBS screening. A) Representative signal curves produced by CRISPR-GBS. A positive control (red), a negative control (black), and 85 clinical samples (blue) are shown with distinct curve patterns (take-off vs. flat). B) Fold-change values by CRISPR-GBS obtained from our prospective cohort: positive (with take-off signal curves in red) and negative (flat curves in blue). A cutoff of 3.5 was set and is indicated in black dashed line. GBS, group B Streptococcus. Lines from the bottom to the top of box-and-whisker plots refer to minimum, first quartile (Q1), median, third quartile (Q3), and maximum number of the dataset.

Figure 3. Determination of assay cutoff for CRISPR-based diagnostic for rapid GBS screening. A) Representative signal curves produced by CRISPR-GBS. A positive control (red), a negative control (black), and 85 clinical samples (blue) are shown with distinct curve patterns (take-off vs. flat). B) Fold-change values by CRISPR-GBS obtained from our prospective cohort: positive (with take-off signal curves in red) and negative (flat curves in blue). A cutoff of 3.5 was set and is indicated in black dashed line. GBS, group B Streptococcus. Lines from the bottom to the top of box-and-whisker plots refer to minimum, first quartile (Q1), median, third quartile (Q3), and maximum number of the dataset.

Main Article

1These authors contributed equally to this article.

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Page updated: August 25, 2021
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