Figure 2. Phylogenetic tree based on the 100-kDa–like protein partial gene sequences of Histoplasma capsulatum from Antarctica. The evolutionary history was inferred by using the maximum-likelihood method in in MEGA X software (https://www.megasoftware.net). This analysis involved 46 sequences: 5 from Antarctica samples and 41 representing geographic lineages of H. capsulatum in addition to the closest non-Histoplasma sequences (Blastomyces spp.) downloaded from GenBank (accession numbers shown). The bootstrap percentage of trees in which the associated taxa clustered together is shown next to the branches. Scale bar indicates the number of substitutions per site.