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Volume 28, Number 11—November 2022

Molecular Detection of Haplorchis pumilio Eggs in Schoolchildren, Kome Island, Lake Victoria, Tanzania

Hyejoo Shin1, Bong-Kwang Jung1, Seungwan Ryoo, Sooji Hong, Heonwoo Jeong, Hoo-Gn Jeoung, Sunhye Kim, Sun Kim, Min-Jae Kim, Hansol Park, Keeseon S. Eom, Godfrey M. Kaatano, and Jong-Yil ChaiComments to Author 
Author affiliations: Korea Association of Health Promotion, Seoul, South Korea (H. Shin, B.-K. Jung, S. Ryoo, S. Hong, H. Jeong, H.-G. Jeoung); Good Neighbors International, Seoul (Sunhye Kim, Sun Kim); Asan Medical Center, University of Ulsan College of Medicine, Seoul (M.-J. Kim); Parasite Resource Bank, Chungbuk National University School of Medicine, Cheongju, South Korea (H. Park, K.-S. Eom); National Institute for Medical Research, Mwanza, Tanzania (G.M. Kaatano); Seoul National University College of Medicine, Seoul (J.-Y. Chai)

Cite This Article


A survey of intestinal helminths targeting 1,440 schoolchildren in 12 primary schools on Kome Island (Lake Victoria), Tanzania, revealed small trematode eggs in 19 children (1.3%), seemingly of a species of Haplorchis or Heterophyes. The eggs were molecularly confirmed to be Haplorchis pumilio on the basis of 18S and 28S rDNA sequences.

Figure 1

Geographic distribution and imaging from study of Haplorchis pumilio eggs in schoolchildren, Kome Island, Lake Victoria, Tanzania. A) Locations of 12 primary schools (P1–P12) surveyed on Kome Island. Inset shows location of Kome Island. B–C) Small trematode eggs (30.0–30.2 µm long and 16.5–16.6 µm wide) detected in schoolchildren, yellowish-brown in color, oval, and operculate with a thick shell and prominent (B) or less prominent shoulder rims (C). Scale bar = 25 µm.

Figure 1. Geographic distribution and imaging from study of Haplorchis pumilioeggs in schoolchildren, Kome Island, Lake Victoria, Tanzania. A) Locations of 12 primary schools (P1–P12) surveyed on Kome Island. Inset...

Haplorchis pumilio, a species of the zoonotic minute intestinal flukes belonging to the family Heterophyidae, was first discovered in the small intestines of birds and mammals in Egypt (1). Infection with this fluke also occurs in humans through the consumption of raw or improperly cooked fish harboring the metacercariae. Abdominal pain, diarrhea, lethargy, anorexia, malabsorption, and weight loss are the possible clinical symptoms (2). This fluke is widely distributed geographically from Africa to Asia, Australia, and the Americas (1). However, human infections were reported in only 5 countries in Africa and Asia: Egypt, China, Laos, Thailand, and Vietnam (1). We recently surveyed the prevalence of intestinal helminths among schoolchildren in 12 primary schools on Kome Island, Lake Victoria, Tanzania (Figure 1, panel A). We detected a low-grade prevalence of an apparent species of Haplorchis or Heterophyes by the recovery of eggs in fecal samples. We used molecular methods to confirm the eggs to be H. pumilio on the basis of 18S and 28S rDNA gene sequences.

The Study

An international collaborative project between South Korea and Tanzania named “Rapid assessment of schistosomiasis and soil-transmitted helminthiases on Kome Island, Buchosa District, northwestern Tanzania” was implemented during 2020–2022. This project was approved by the Ethics Committee of the Korea Association of Health Promotion, Seoul, South Korea (IRB no. 130750-202009-HR-019). Fecal examinations were performed on 1,440 schoolchildren in 12 primary schools by using the Kato-Katz thick smear technique.

The number of overall helminth egg–positive cases was 631/1,440 (43.8%): Schistosoma mansoni (564 [39.2%]), Trichuris trichiura (42 [2.9%]), Ancylostoma duodenale or Necator americanus (27 [1.9%]), small trematode eggs (STE) (19 [1.3%]), Enterobius vermicularis (16 [1.1%]), and others (unidentified) (7 [0.5%]). The STE were operculate, oval, yellowish-brown in color, 29.0–31.6 (mean 30.4) µm long, and 14.8–17.6 (mean 16.5) µm wide (n = 6). They seemed to be the eggs of a Haplorchis or Heterophyes species (Figure 1, panels B and C). The STE-positive fecal samples were preserved in 100% ethanol for molecular analysis.

We extracted DNA from 20 mg of the fecal sediment by using the DNeasy Tissue and Blood kit (QIAGEN, after a modified formalin-ether concentration method in which formalin was replaced with water. The sediment was washed several times with distilled water. We performed PCR targeting the 18S and 28S rDNA of Haplorchis species using the primers we designed on the basis of the reported nucleotide sequences of Haplorchis and Heterophyes in GenBank (Table 1). We conducted PCR in a final volume of 20 µL using 5x PCR Premix (GenomicsOne, The procedure included an initial denaturation at 94°C for 3 min, followed by 40 cycles of denaturation at 94°C for 30 s, annealing at 60°C for 30 s, extension at 72°C for 30 s, and final extension at 72°C for 5 min. The amplicons were electrophoresed in 2.0% agarose gel, and DNA sequencing was performed using the Sanger method (3) by Macrogen Inc. ( We aligned sequences of the amplicons and generated phylogenetic trees with the maximum-likelihood method in MEGA version 7.0 software ( by using the Kimura 2-parameter model with 1,000 bootstrap replications.

Figure 2

Phylogenetic trees of DNA of small trematode eggs from schoolchildren, Kome Island, Lake Victoria, Tanzania, in comparison with reference sequences of heterophyid (Haplorchis pumilio and others) and opisthorchiid trematodes, based on 18S (A) and 28S rDNA (B) sequences. The trees were constructed using the maximum-likelihood method based on the Kimura 2-parameter model and viewed by the MEGA 7.0 program ( GenBank accession numbers are indicated. Scale bars indicate nucleotide substitutions per site.

Figure 2. Phylogenetic trees of DNA of small trematode eggs from schoolchildren, Kome Island, Lake Victoria, Tanzania, in comparison with reference sequences of heterophyid (Haplorchis pumilioand others) and opisthorchiid trematodes,...

Sequences of the 18S region of our samples (n = 4) were 100% identical to the 18S rDNA gene of H. pumilio in GenBank (accession nos. AY245706 and HM004196) (Table 2; Figure 2, panel A). However, only 95.0% identity was found between our samples and Haplorchis taichui (accession no. AY245705) and 98.9% was found between our samples and Haplorchis yokogawai (accession no. HM004208). However, using this gene, comparing our samples with Heterophyes heterophyes was not possible because 18S rDNA sequences of H. heterophyes are not available in GenBank.

Sequences of the 28S region of our samples (n = 3) were 99.1%–100% identical to the 28S rDNA gene of H. pumilio in GenBank (accession nos. MN745941 and MT840091) (Table 2; Figure 2, panel B). However, only 93.7% identity was found between our samples and H. taichui (accession no. OM956185) and 95.8% identity was found between our samples and H. yokogawai (accession no. HM004192). H. heterophyes (accession no. KU559554) appeared to be far from our samples (Figure 2, panel B) showing a sequence identity of only 86.9% (Table 2). Thus, we could confirm that our samples were mostly the eggs of H. pumilio and that Kome Island is a low-grade endemic area of H. pumilio infection among schoolchildren. However, possibilities remain for mixed infections with other heterophyid species (low worm loads and not detected by PCR).


Taxonomically, in the genus Haplorchis, a total of 9 species have been known to be valid (1). Among them, 4 species are recognized to be zoonotic: H. pumilio, H. taichui, H. yokogawai, and H. vanissimus (1). Natural human infection with H. pumilio flukes was first documented in Egypt in 1977 in a 9-year-old child passing diarrheic stools (4). A vital snail species for H. pumilio flukes is Melanoides tuberculata in Egypt, Taiwan, India, Peru, and Brazil (1,5). Their metacercariae are detected in various species of freshwater or brackish water fish, including Mugil sp., Tilapia sp., and Bagrus bayad (1,6).

In Africa, with the exception of Egypt (an H. pumilio fluke–endemic area), the distribution of H. pumilio flukes has been rarely reported. In Kenya, H. pumilio cercariae were confirmed molecularly recently in M. tuberculata snails; a high positive rate of 69.4% was found in the northernmost area of Lake Victoria, in Kenya (7). However, human H. pumilio infection in sub-Saharan Africa countries, including Kenya, has not been reported. In São Tomé and Principe, a sub-Sahara country off the west coast of Africa, eggs of Heterophyidae, which are very similar to Metagonimus yokogawai, were found in 28.2% of 1,050 human fecal samples in 1987, but their species could not be identified (8). Those eggs were 22.2–27.7 × 17.0–21.0 μm in size and had a thick wall and a difficult-to-see operculum (8); they were markedly different from the eggs of Haplorchis or Heterophyes spp (1). Of note, a zoonotic liver fluke species, Opisthorchis felineus, was found in dogs and cats in New Bussa, Nigeria (9); however, this species has never been found to distribute around Lake Victoria.

On the Lake Victoria basin, schistosomiasis and soil-transmitted helminthiases have been acknowledged as major public health problems (10), whereas intestinal fluke infections have been poorly studied. In this study, we detected a low-grade endemicity of H. pumilio infection on Kome Island, Lake Victoria, Tanzania. It remains unclear if human H. pumilio infection has been endemic on Kome Island unnoticed for a long time or was introduced recently. These 2 possibilities should be investigated.

Surveyed schoolchildren on Kome Island had no history of international travel, including to Asia, South America, Egypt, and Kenya, where the H. pumilio fluke is endemic. Therefore, the source of infection in our cases seems to be the fish host caught around Kome Island. In Lake Victoria, 3 fish species are known to predominate, and one of them is Nile tilapia (Oreochromis niloticus), which is a fish host for H. pumilio flukes (1,11,12). Nile tilapia is popularly eaten on Kome Island and is highly suggested as the source of infection in our cases. Studies are required to determine the existence of the life cycle of H. pumilio flukes on and around Kome Island and clarify the public health importance of H. pumilio infection in this area.

Ms. Shin and Dr. Jung are research associates at the MediCheck Research Institute, Korea Association of Health Promotion, Seoul, South Korea. Their major research interests are molecular analyses of foodborne zoonotic parasites, including heterophyids, echinostomes, Fasciola sp., and Toxoplasma gondii.



We thank the staff of the International Cooperation Department of the Korea Association of Health Promotion and Good Neighbors International for their help in this study. We are also grateful to the members of the National Institute of Medical Research, Mwanza, Tanzania, who helped with this study.



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Cite This Article

DOI: 10.3201/eid2811.220653

1These authors contributed equally to this article.

Table of Contents – Volume 28, Number 11—November 2022

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Jong-Yil Chai, Department of Tropical Medicine and Parasitology, Seoul National University College of Medicine, Seoul 03080, South Korea

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Page created: September 07, 2022
Page updated: October 24, 2022
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