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Volume 28, Number 11—November 2022
Dispatch

Molecular Detection of Haplorchis pumilio Eggs in Schoolchildren, Kome Island, Lake Victoria, Tanzania

Hyejoo Shin1, Bong-Kwang Jung1, Seungwan Ryoo, Sooji Hong, Heonwoo Jeong, Hoo-Gn Jeoung, Sunhye Kim, Sun Kim, Min-Jae Kim, Hansol Park, Keeseon S. Eom, Godfrey M. Kaatano, and Jong-Yil ChaiComments to Author 
Author affiliations: Korea Association of Health Promotion, Seoul, South Korea (H. Shin, B.-K. Jung, S. Ryoo, S. Hong, H. Jeong, H.-G. Jeoung); Good Neighbors International, Seoul (Sunhye Kim, Sun Kim); Asan Medical Center, University of Ulsan College of Medicine, Seoul (M.-J. Kim); Parasite Resource Bank, Chungbuk National University School of Medicine, Cheongju, South Korea (H. Park, K.-S. Eom); National Institute for Medical Research, Mwanza, Tanzania (G.M. Kaatano); Seoul National University College of Medicine, Seoul (J.-Y. Chai)

Main Article

Table 1

Primers used for DNA amplification in study of Haplorchis pumilio eggs in schoolchildren, Kome Island, Lake Victoria, Tanzania*

Target gene Primer Sequence, 5′ → 3′ Length, bp
18S rDNA 18S 1F ATACGGGACTCGTTAGAGGC 504
18S 1R
 TACAAATGCCCCCGTCTGTC
28S rDNA 28S 1F AGTGAACAGGGAAAAGCCCAG 897
28S 1R TCAGGTGGAAAGTCTACCGC
28S 2F ATAGCGAACAAGTACCGTGAGG
28S 2R ACATGTTACTCTCCTTGGTCCG 659

*Primers were designed using Geneious primer design software (https://www.geneious.com) based on the 18S rDNA sequences of Haplorchis pumilio (GenBank accession no. AY245706) and H. taichui (accession no. AY245705) and the 28S rDNA sequences of H. pumilio (accession no. HM004173) and Heterophyes heterophyes (accession no. KU559553).

Main Article

1These authors contributed equally to this article.

Page created: September 07, 2022
Page updated: October 24, 2022
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