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Volume 28, Number 5—May 2022
Dispatch

Novel Hendra Virus Variant Circulating in Black Flying Foxes and Grey-Headed Flying Foxes, Australia

Alison J. Peel1Comments to Author , Claude Kwe Yinda1, Edward J. Annand, Adrienne S. Dale, Peggy Eby, John-Sebastian Eden, Devin N. Jones, Maureen K. Kessler, Tamika J. Lunn, Tim Pearson, Jonathan E. Schulz, Ina L. Smith, Vincent J. Munster2, Raina K. Plowright2, and Bat One Health Group3
Author affiliations: Griffith University Centre for Planetary Health and Food Security, Nathan, Queensland, Australia (A.J. Peel, P. Eby, T.J. Lunn); National Institutes of Health, Hamilton, Montana, USA (C.K. Yinda, J.E. Schulz, V.J. Munster); EquiEpiVet, Aireys Inlet, Victoria, Australia (E.J. Annand); Department of Agriculture, Water, and the Environment, Canberra, Australian Capital Territory, Australia (E.J. Annand); University of Sydney, Sydney, New South Wales, Australia (E.J. Annand, J.-S. Eden); Texas Tech University, Lubbock, Texas, USA (A.S. Dale); University of New South Wales, Sydney (P. Eby); Montana State University, Bozeman, Montana, USA (D.N. Jones, M.K. Kessler, R.K. Plowright); Bellingen, New South Wales, Australia (T. Pearson); CSIRO, Black Mountain, Australian Capital Territory, Australia (I.L. Smith)

Main Article

Table 1

Primers and probes used in PCR for study of novel Hendra virus variant circulating in black and grey-headed flying foxes, Australia*

Target Primers and Probes Reference
HeV-g1 P gene F: 5′-CCCAACCAAGAAAGCAAGAG This study
R: 5′-TTCATTCCTCGTGACAGCAC
P: 5′-TTACTGCGGAGAATGTCCAACTGAGTG
HeV-g1 M gene F: 5′-CTTCGACAAAGACGGAACCAA (2)
R: 5′ TGGCATCTTTCATGCTCCATCTCGG
P: 5′ CCAGCTCGTCGGACAAAATT
HeV-g2 M gene F: 5′ TCTCGACAAGGACGGAGCTAA (3)
R: 5′ CCGGCTCGTCGAACAAAATT
P: 5′ TGGCATCCTTCATGCTTCACCTTGG
Partial cytochrome b gene F: 5′-CGAAGCTTGATATGAAAAACCATCGTTG (10,11)
R: 5′ AACTGCAGCCCCTCAGAATGATATTTGTCCTCA
*F, forward; R, reverse; P, probe.

Main Article

References
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  12. Plowright  RK, Eby  P, Hudson  PJ, Smith  IL, Westcott  D, Bryden  WL, et al. Ecological dynamics of emerging bat virus spillover. Proc Biol Sci. 2015;282:20142124. DOIPubMedGoogle Scholar
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1These authors contributed equally to this article.

2These senior authors contributed equally to this article.

3Members of Bat One Health are listed at the end of this article.

Page created: March 07, 2022
Page updated: April 19, 2022
Page reviewed: April 19, 2022
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