Volume 28, Number 5—May 2022
Dispatch
Novel Hendra Virus Variant Circulating in Black Flying Foxes and Grey-Headed Flying Foxes, Australia
Table 1
Primers and probes used in PCR for study of novel Hendra virus variant circulating in black and grey-headed flying foxes, Australia*
Target | Primers and Probes | Reference |
---|---|---|
HeV-g1 P gene | F: 5′-CCCAACCAAGAAAGCAAGAG | This study |
R: 5′-TTCATTCCTCGTGACAGCAC | ||
P: 5′-TTACTGCGGAGAATGTCCAACTGAGTG |
||
HeV-g1 M gene | F: 5′-CTTCGACAAAGACGGAACCAA | (2) |
R: 5′ TGGCATCTTTCATGCTCCATCTCGG | ||
P: 5′ CCAGCTCGTCGGACAAAATT |
||
HeV-g2 M gene | F: 5′ TCTCGACAAGGACGGAGCTAA | (3) |
R: 5′ CCGGCTCGTCGAACAAAATT | ||
P: 5′ TGGCATCCTTCATGCTTCACCTTGG |
||
Partial cytochrome b gene | F: 5′-CGAAGCTTGATATGAAAAACCATCGTTG | (10,11) |
R: 5′ AACTGCAGCCCCTCAGAATGATATTTGTCCTCA |
||
*F, forward; R, reverse; P, probe. |
References
- Playford EG, McCall B, Smith G, Slinko V, Allen G, Smith I, et al. Human Hendra virus encephalitis associated with equine outbreak, Australia, 2008. Emerg Infect Dis. 2010;16:219–23. DOIPubMedGoogle Scholar
- Smith IL, Halpin K, Warrilow D, Smith GA. Development of a fluorogenic RT-PCR assay (TaqMan) for the detection of Hendra virus. J Virol Methods. 2001;98:33–40. DOIPubMedGoogle Scholar
- Annand EJ, Horsburgh BA, Xu K, Reid PA, Poole B, de Kantzow MC, et al. Novel Hendra virus variant detected by sentinel surveillance of Australian horses. Emerg Infect Dis. 2022;28:693–704. DOIPubMedGoogle Scholar
- Field H, Jordan D, Edson D, Morris S, Melville D, Parry-Jones K, et al. Spatiotemporal aspects of Hendra virus infection in pteropid bats (flying-foxes) in eastern Australia. PLoS One. 2015;10:
e0144055 . DOIPubMedGoogle Scholar - Tong S, Chern S-WW, Li Y, Pallansch MA, Anderson LJ. Sensitive and broadly reactive reverse transcription-PCR assays to detect novel paramyxoviruses. J Clin Microbiol. 2008;46:2652–8. DOIPubMedGoogle Scholar
- Wang J, Anderson DE, Halpin K, Hong X, Chen H, Walker S, et al. A new Hendra virus genotype found in Australian flying foxes. Virol J. 2021;18:197. DOIPubMedGoogle Scholar
- Edson D, Field H, McMichael L, Vidgen M, Goldspink L, Broos A, et al. Routes of Hendra virus excretion in naturally-infected flying-foxes: implications for viral transmission and spillover risk. PLoS One. 2015;10:
e0140670 . DOIPubMedGoogle Scholar - Smith I, Broos A, de Jong C, Zeddeman A, Smith C, Smith G, et al. Identifying Hendra virus diversity in pteropid bats. PLoS One. 2011;6:
e25275 . DOIPubMedGoogle Scholar - Kocher TD, Thomas WK, Meyer A, Edwards SV, Pääbo S, Villablanca FX, et al. Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc Natl Acad Sci U S A. 1989;86:6196–200. DOIPubMedGoogle Scholar
- Hsieh H-M, Chiang H-L, Tsai L-C, Lai S-Y, Huang N-E, Linacre A, et al. Cytochrome b gene for species identification of the conservation animals. Forensic Sci Int. 2001;122:7–18. DOIPubMedGoogle Scholar
- Plowright RK, Eby P, Hudson PJ, Smith IL, Westcott D, Bryden WL, et al. Ecological dynamics of emerging bat virus spillover. Proc Biol Sci. 2015;282:
20142124 . DOIPubMedGoogle Scholar - Lunney D, Richards G. Dickman C Pteropus poliocephalus. The IUCN Red List of Threatened Species [cited 2021 Nov 15]. https://www.iucnredlist.org/species/18751/22085511
1These authors contributed equally to this article.
2These senior authors contributed equally to this article.
3Members of Bat One Health are listed at the end of this article.