Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 28, Number 9—September 2022
Research

Detection of Endosymbiont Candidatus Midichloria mitochondrii and Tickborne Pathogens in Humans Exposed to Tick Bites, Italy

Giovanni Sgroi1, Roberta Iatta1, Piero Lovreglio, Angela Stufano, Younes Laidoudi, Jairo Alfonso Mendoza-Roldan, Marcos Antonio Bezerra-Santos, Vincenzo Veneziano, Francesco Di Gennaro, Annalisa Saracino, Maria Chironna, Claudio Bandi, and Domenico OtrantoComments to Author 
Author affiliations: University of Bari Aldo Moro, Bari, Italy (G. Sgroi, R. Iatta, P. Lovreglio, A. Stufano, Y. Laidoudi, J.A. Mendoza-Roldan, M.A. Bezerra-Santos, F. Di Gennaro, A. Saracino, M. Chironna, D. Otranto); University of Naples Federico II, Naples, Italy (V. Veneziano); Osservatorio Faunistico Venatorio, Naples (V. Veneziano); University of Milan Statale, Milan, Italy (C. Bandi); Bu-Ali Sina University, Hamedan, Iran (D. Otranto)

Main Article

Figure 2

Maximum-likelihood phylogenic tree of endosymbiont Candidatus Midichloria mitochondrii clades detected with tickborne pathogens in humans exposed to tick bites, Italy, 2021. The tree corresponds to the IQ-TREE (http://www.iqtree.org) inferred from 38 partial (202 bp) DNA sequences with 28.7% of informative sites by using the Kimura 3-parameter model with empirical frequencies plus regression (TPM3+F+R2) model under 1,000 bootstrap replicates and maximum-likelihood method. Accession numbers and species name are indicated at the tip of each branch. Bold blue text indicates sequences amplified in the study area. The tree includes 123 query sequences representing all 16S rRNA entries from GenBank (blue circles on left) placed at the branch and leaf nodes by using the APPLES algorithm (https://github.com/balabanmetin/apples). The ClustalX (http://www.clustal.org/clustal2) sequence alignment viewer of the informative sites from the 16S rRNA alignment and their 50% consensus are shown. Colored column at far right denotes taxonomic annotation; purple indicates clade A, blue indicates clade B, green indicates clade C, yellow indicates clade D, green indicates clade E, gray indicates outgroup. Ca., Candidatus.

Figure 2. Maximum-likelihood phylogenic tree of endosymbiont Candidatus Midichloria mitochondrii clades detected with tickborne pathogens in humans exposed to tick bites, Italy, 2021. The tree corresponds to the IQ-TREE (http://www.iqtree.org) inferred from 38 partial (202 bp) DNA sequences with 28.7% of informative sites by using the Kimura 3-parameter model with empirical frequencies plus regression (TPM3+F+R2) model under 1,000 bootstrap replicates and maximum-likelihood method. Accession numbers and species name are indicated at the tip of each branch. Bold blue text indicates sequences amplified in the study area. The tree includes 123 query sequences representing all 16S rRNA entries from GenBank (blue circles on left) placed at the branch and leaf nodes by using the APPLES algorithm (https://github.com/balabanmetin/apples). The ClustalX (http://www.clustal.org/clustal2) sequence alignment viewer of the informative sites from the 16S rRNA alignment and their 50% consensus are shown. Colored column at far right denotes taxonomic annotation; purple indicates clade A, blue indicates clade B, green indicates clade C, yellow indicates clade D, green indicates clade E, gray indicates outgroup. Ca., Candidatus.

Main Article

1These authors contributed equally to this article.

Page created: July 27, 2022
Page updated: August 19, 2022
Page reviewed: August 19, 2022
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external