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Volume 29, Number 10—October 2023
Dispatch

Pseudomonas aeruginosa High-Risk Sequence Type 463 Co-Producing KPC-2 and AFM-1 Carbapenemases, China, 2020–2022

Piaopiao Zhang1, Wenhao Wu1, Nanfei Wang, Haiting Feng, Jie Wang, Fang Wang, Yan Zhang, Hongchao Chen, Qing Yang, Yan Jiang2, and Tingting Qu2Comments to Author 
Author affiliations: The First Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China (P. Zhang, W. Wu, N. Wang, H. Feng, J. Wang, F. Wang, Y. Zhang, H. Chen, Q. Yang, T. Qu); Zhejiang University School of Medicine Sir Run Run Shaw Hospital, Hangzhou (Y. Jiang); Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou (Y. Jiang)

Main Article

Figure 2

Desiccation tolerance and virulence analyses of Pseudomonas aeruginosa high-risk sequence type 463 co-producing KPC-2 and AFM-1 carbapenemases, China, 2020–2022. A) Carbapenem-resistant P. aeruginosa strains ZY94 and ZY1214 isolated from hospital patients in China were evaluated for desiccation tolerance. Results are given as mean (SD) CFU/mL for each isolate before and after 8 days of desiccation. Laboratory reference strain PAO1 and P. aeruginosa NDTH6412, which belongs to high-risk international sequence type 235, were used as controls. B) Virulence was determined by using Kaplan-Meier survival curves of mice intraperitoneally challenged with 1 × 107 CFUs of strain ZY94 or ZY1214, hypervirulent strain PA14, or nonvirulent P. aeruginosa strain ATCC9027. Mice (10 per group) were monitored for 5 days and the number of dead mice was assessed each day. Mantel-Cox log rank tests were used to calculate p values for survival curve comparisons between the different strains. AFM, Alcaligenes faecalis metallo-β-lactamase; CFUs, colony forming units; KPC, Klebsiella pneumoniae carbapenemase.

Figure 2. Desiccation tolerance and virulence analyses of Pseudomonas aeruginosa high-risk sequence type 463 co-producing KPC-2 and AFM-1 carbapenemases, China, 2020–2022. A) Carbapenem-resistant P. aeruginosa strains ZY94 and ZY1214 isolated from hospital patients in China were evaluated for desiccation tolerance. Results are given as mean (SD) CFU/mL for each isolate before and after 8 days of desiccation. Laboratory reference strain PAO1 and P. aeruginosa NDTH6412, which belongs to high-risk international sequence type 235, were used as controls. B) Virulence was determined by using Kaplan-Meier survival curves of mice intraperitoneally challenged with 1 × 107 CFUs of strain ZY94 or ZY1214, hypervirulent strain PA14, or nonvirulent P. aeruginosa strain ATCC9027. Mice (10 per group) were monitored for 5 days and the number of dead mice was assessed each day. Mantel-Cox log rank tests were used to calculate p values for survival curve comparisons between the different strains. AFM, Alcaligenes faecalis metallo-β-lactamase; CFUs, colony forming units; KPC, Klebsiella pneumoniae carbapenemase.

Main Article

1These authors contributed equally to this article.

2These senior authors contributed equally to this article.

Page created: August 08, 2023
Page updated: September 20, 2023
Page reviewed: September 20, 2023
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