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Volume 29, Number 11—November 2023

Research

Neurologic Effects of SARS-CoV-2 Transmitted among Dogs

Dong-Hwi Kim1, Da-Yoon Kim1, Kyu-Sung Kim1, Sang-Hoon Han, Hyeon-Jeong Go, Jae-Hyeong Kim, Kyu-Beom Lim, Dong-Hun Lee, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Yang-Kyu Choi, Yeun-Kyung Shin, Oh-Kyu Kwon, Do-Geun KimComments to Author , and In-Soo ChoiComments to Author 
Author affiliations: Konkuk University, Seoul, South Korea (D.-H. Kim, D.-Y. Kim, S.-H. Han, H.-J. Go, J.-H. Kim, K.-B. Lim, D.-H. Lee, J.-B. Lee, S.-Y. Park, C.-S. Song, S.-W. Lee, Y.-K. Choi, I.-S. Choi); Korea Brain Research Institute, Daegu, South Korea (K.-S. Kim, D.-G. Kim); Daegu Gyeongbuk Institute of Science and Technology, Daegu (K.-S. Kim, D.-G. Kim); Animal and Plant Quarantine Agency, Gimcheon, South Korea (Y.-K. Shin, O.-K. Kwon); Konkuk University Zoonotic Diseases Research Center, Seoul (D.-H. Lee, J.-B. Lee, S.-Y. Park, C.-S. Song, S.-W. Lee, I.-S. Choi); KU Center for Animal Blood Medical Science, Seoul (I.-S. Choi)

Main Article

Figure 4

SARS-CoV-2 induces activation of microglial cells in the brain white matter in a region-specific manner in SARS-CoV-2–infected and contact dogs in study of the neurologic effects of SARS-CoV-2 transmitted among dogs. A) Representative fluorescent images of glial fibrillary acidic protein (activation astrocyte marker, green) staining of canine brain sections derived from SARS-CoV-2–infected and contact groups at early and late days after infection. Scale bars indicate 200 μm; in insets, 50 μm. B) Representative fluorescent images and statistical results of Iba-1 (a marker of microglia; green) staining of canine brain white matter sections derived from SARS-CoV-2–infected and contact dogs at early and late dpi. Scale bars indicate 200 μm; in insets, 50 μm. C) Representative fluorescent images and statistical results of Iba-1 (a marker of microglia, green) staining of canine brain gray matter sections derived from SARS-CoV-2–infected and contact dogs at early and late dpi. Scale bars indicate 200 μm; in insets, 50 μm. Statistical significance was determined using a 1-way analysis of variance. Data in graphs are presented as means ±SEM.

Figure 4. SARS-CoV-2 induces activation of microglial cells in the brain white matter in a region-specific manner in SARS-CoV-2–infected and contact dogs in study of the neurologic effects of SARS-CoV-2 transmitted among dogs. A) Representative fluorescent images of glial fibrillary acidic protein (activation astrocyte marker, green) staining of canine brain sections derived from SARS-CoV-2–infected and contact groups at early and late days after infection. Scale bars indicate 200 μm; in insets, 50 μm. B) Representative fluorescent images and statistical results of Iba-1 (a marker of microglia; green) staining of canine brain white matter sections derived from SARS-CoV-2–infected and contact dogs at early and late dpi. Scale bars indicate 200 μm; in insets, 50 μm. C) Representative fluorescent images and statistical results of Iba-1 (a marker of microglia, green) staining of canine brain gray matter sections derived from SARS-CoV-2–infected and contact dogs at early and late dpi. Scale bars indicate 200 μm; in insets, 50 μm. Statistical significance was determined using a 1-way analysis of variance. Data in graphs are presented as means ±SEM.

Main Article

1These authors contributed equally to this article.

Page created: September 18, 2023
Page updated: October 23, 2023
Page reviewed: October 23, 2023
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