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Volume 29, Number 11—November 2023
Research

Neurologic Effects of SARS-CoV-2 Transmitted among Dogs

Dong-Hwi Kim1, Da-Yoon Kim1, Kyu-Sung Kim1, Sang-Hoon Han, Hyeon-Jeong Go, Jae-Hyeong Kim, Kyu-Beom Lim, Dong-Hun Lee, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Yang-Kyu Choi, Yeun-Kyung Shin, Oh-Kyu Kwon, Do-Geun KimComments to Author , and In-Soo ChoiComments to Author 
Author affiliations: Konkuk University, Seoul, South Korea (D.-H. Kim, D.-Y. Kim, S.-H. Han, H.-J. Go, J.-H. Kim, K.-B. Lim, D.-H. Lee, J.-B. Lee, S.-Y. Park, C.-S. Song, S.-W. Lee, Y.-K. Choi, I.-S. Choi); Korea Brain Research Institute, Daegu, South Korea (K.-S. Kim, D.-G. Kim); Daegu Gyeongbuk Institute of Science and Technology, Daegu (K.-S. Kim, D.-G. Kim); Animal and Plant Quarantine Agency, Gimcheon, South Korea (Y.-K. Shin, O.-K. Kwon); Konkuk University Zoonotic Diseases Research Center, Seoul (D.-H. Lee, J.-B. Lee, S.-Y. Park, C.-S. Song, S.-W. Lee, I.-S. Choi); KU Center for Animal Blood Medical Science, Seoul (I.-S. Choi)

Main Article

Figure 5

SARS-CoV-2 infection causing perivascular demyelination in the brain in dogs in study of the neurologic effects of SARS-CoV-2 transmitted among dogs. A, B) Representative fluorescent images and statistical analysis results of NFL (a marker of neurofilament light chain; gray) staining of canine brain white matter sections derived from SARS-CoV-2 infected and contact dogs at early and late dpi. Scale bar indicates 200 μm. Overall images from infected dogs demonstrate irregular axonal morphology compared with that of control dogs. Single-layer slice images. Scale bar indicates 20 μm. C, D) Representative fluorescent images and statistical analysis results of myelin (fluomyelin; green) and DAPI (blue) staining of canine brain white matter sections derived from SARS-CoV-2–infected and contact dogs at early and late dpi. Scale bar indicates 200 μm. E) Representative fluorescent images of p-tau (red) staining of canine brain gray matter sections derived from SARS-CoV-2–infected and contact dogs at early and late dpi. Scale bar indicates 200 μm. F–H) Representative fluorescent images (F) and statistical analysis results (G, H) of NeuN (a marker of neuron; green) staining of canine brain gray matter sections derived from SARS-CoV-2–infected and contact dogs at early (G) and late (H) infection. Scale bar indicates 200 μm. Statistical significance was determined using a 1-way analysis of variance. Data are presented as mean ±SEM. NS, not significant.

Figure 5. SARS-CoV-2 infection causing perivascular demyelination in the brain in dogs in study of the neurologic effects of SARS-CoV-2 transmitted among dogs. A, B) Representative fluorescent images and statistical analysis results of NFL (a marker of neurofilament light chain; gray) staining of canine brain white matter sections derived from SARS-CoV-2 infected and contact dogs at early and late dpi. Scale bar indicates 200 μm. Overall images from infected dogs demonstrate irregular axonal morphology compared with that of control dogs. Single-layer slice images. Scale bar indicates 20 μm. C, D) Representative fluorescent images and statistical analysis results of myelin (fluomyelin; green) and DAPI (blue) staining of canine brain white matter sections derived from SARS-CoV-2–infected and contact dogs at early and late dpi. Scale bar indicates 200 μm. E) Representative fluorescent images of p-tau (red) staining of canine brain gray matter sections derived from SARS-CoV-2–infected and contact dogs at early and late dpi. Scale bar indicates 200 μm. F–H) Representative fluorescent images (F) and statistical analysis results (G, H) of NeuN (a marker of neuron; green) staining of canine brain gray matter sections derived from SARS-CoV-2–infected and contact dogs at early (G) and late (H) infection. Scale bar indicates 200 μm. Statistical significance was determined using a 1-way analysis of variance. Data are presented as mean ±SEM. NS, not significant.

Main Article

1These authors contributed equally to this article.

Page created: September 18, 2023
Page updated: October 23, 2023
Page reviewed: October 23, 2023
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