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Volume 29, Number 12—December 2023
Research

Detection of Anopheles stephensi Mosquitoes by Molecular Surveillance, Kenya

Eric O. OchomoComments to Author , Sylvia Milanoi, Bernard Abong’o, Brenda Onyango, Margaret Muchoki, Diana Omoke, Evelyn Olanga, Laban Njoroge, Elijah Omondi Juma, James Dan Otieno, Damaris Matoke-Muhia, Luna Kamau, Cristina Rafferty, John E. Gimnig, Mildred Shieshia, Daniel Wacira, Joseph Mwangangi, Marta Maia, Charles Chege, Ahmeddin Omar, Martin K. Rono, Lucy Abel, Wendy Prudhomme O’Meara, Andrew Obala, Charles Mbogo, and Lenson Kariuki
Author affiliations: Kenya Medical Research Institute, Nairobi, Kenya (E.O. Ochomo, S. Milanoi, B. Abong’o, B. Onyango, M. Muchoki, D. Omoke, D. Matoke-Muhia, L. Kamau, J. Mwangangi, M. Maia, M.K. Rono, C. Mbogo); PMI Kinga Malaria Project, Abt Associates Inc., Kisumu, Kenya (E. Olanga); National Museums of Kenya, Nairobi (L. Njoroge); Pan-African Mosquito Control Association, Nairobi (E.O. Juma, D. Matoke-Muhia, C. Mbogo); World Health Organization, Kenya Country Office, Nairobi (J.D. Otieno); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (C. Rafferty, J.E. Gimnig); US President’s Malaria Initiative, Washington, DC, USA (C. Rafferty, J.E. Gimnig, M. Shieshia, D. Wacira); University of Oxford, Oxford, UK (M. Maia); KEMRI-Wellcome Trust, Kilifi, Kenya (M. Maia, M.K. Rono); Division of National Malaria Program, Ministry of Health, Nairobi (C. Chege, A. Omar); Moi University, Eldoret (W.P. O’Meara, A. Obala); AMPATH, Eldoret, Kenya (L. Abel); Duke University, Durham, North Carolina, USA (W.P. O’Meara, L. Kariuki)

Main Article

Table 2

Numbers of mosquito larvae or adults used for PCR to identify Anopheles stephensi mosquitoes, Kenya*

Subcounty Total Anopheles larvae collected Larvae used for PCR
Adults used for PCR
Sample size An. stephensi Unamplified Sample size An. stephensi Unamplified
Saku 42 9 6 3 33 8 25
Laisamis 17 2 1 1 15 8 7
Moyale 51 51 2 27 NA NA NA
Turkana Central† 193 193 5 188 NA NA NA

*Unamplified samples are those that failed to amplify with the An. stephensi, An. gambiae, and An. funestus PCR protocols. NA, not available. †Including only a single site that was positive for An. stephensi. Five larvae were shipped to KEMRI-Wellcome Trust for sequencing.

Main Article

Page created: October 11, 2023
Page updated: November 18, 2023
Page reviewed: November 18, 2023
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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