Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link

Volume 29, Number 4—April 2023

Dispatch

Ocular Trematodiasis in Children, Sri Lanka

Chandana H. Mallawarachchi, Mangala M. Dissanayake, Sidesh R. Hendavitharana, Saman Senanayake, Nisayuri Gunathilaka, Nilmini T.G.A. Chandrasena, Thishan C. Yahathugoda, Susiji WickramasingheComments to Author , and Nilanthi R. de Silva
Author affiliations: Medical Research Institute, Colombo, Sri Lanka (C.H. Mallawarachchi); District General Hospital, Trincomalee, Sri Lanka (M.M. Dissanayake, N. Gunathilaka); Base Hospital, Kantale, Sri Lanka (S.R. Hendavitharana); National Hospital, Kandy, Sri Lanka (S. Senanayake); University of Kelaniya, Kelaniya, Sri Lanka (N.T.G.A. Chandrasena, N.R. de Silva); University of Ruhuna, Galle, Sri Lanka (T.C. Yahathugoda); University of Peradeniya, Peradeniya, Sri Lanka (S. Wickramasinghe)

Main Article

Figure 2

Phylogenetic analysis of isolates from 2 episcleral nodule isolates from the eyes of pediatric patients in study of ocular trematodiasis in children, Sri Lanka. Genomic DNA was isolated from biopsy samples from 2 patients. PCR was used to target the trematode internal transcribed spacer 2 (ITS2) gene, which was then sequenced. Maximum-likelihood analysis was used to construct a phylogenetic tree containing partial sequences of isolate 1 and 2 (bold font) from Sri Lanka and 24 taxa from GenBank. Partial ITS2 gene sequences were aligned by using MEGA version 10.2.4 software (https://www.megasoftware.net). Numbers near nodes indicate the percentages of 1,000 nonparametric bootstrap pseudoreplicates (>50). GenBank accession numbers are provided for ITS2 gene reference sequences. The sequences for the 2 isolates from this study were deposited in GenBank (accession nos. OP516360 and OP516359). Metagonimus yokogawai (KJ631740) isolated from a cat is included as the outgroup. SL, Sri Lanka. Scale bar indicates nucleotide substitutions per site.

Figure 2. Phylogenetic analysis of isolates from 2 episcleral nodule isolates from the eyes of pediatric patients in study of ocular trematodiasis in children, Sri Lanka. Genomic DNA was isolated from biopsy samples from 2 patients. PCR was used to target the trematode internal transcribed spacer 2 (ITS2) gene, which was then sequenced. Maximum-likelihood analysis was used to construct a phylogenetic tree containing partial sequences of isolate 1 and 2 (bold font) from Sri Lanka and 24 taxa from GenBank. Partial ITS2 gene sequences were aligned by using MEGA version 10.2.4 software (https://www.megasoftware.net). Numbers near nodes indicate the percentages of 1,000 nonparametric bootstrap pseudoreplicates (>50). GenBank accession numbers are provided for ITS2 gene reference sequences. The sequences for the 2 isolates from this study were deposited in GenBank (accession nos. OP516360 and OP516359). Metagonimus yokogawai (KJ631740) isolated from a cat is included as the outgroup. SL, Sri Lanka. Scale bar indicates nucleotide substitutions per site.

Main Article

Page created: January 25, 2023
Page updated: March 21, 2023
Page reviewed: March 21, 2023
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external