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Volume 29, Number 5—May 2023
CME ACTIVITY - Research

Emergence of Erythromycin-Resistant Invasive Group A Streptococcus, West Virginia, USA, 2020–2021

Lillie M. Powell, Soo Jeon Choi, Chloe E. Chipman, Megan E. Grund, P. Rocco LaSala1, and Slawomir Lukomski1Comments to Author 
Author affiliation: West Virginia University, Morgantown, West Virginia, USA

Main Article

Figure 4

Detection of the methyl transferase genes ermA, ermB, and ermT in invasive group A Streptococcus (iGAS) isolates, West Virginia, USA, 2020–2021. A) Distribution of the pRW35-like plasmid among iGAS isolates. Presence of pRW35-like plasmid DNA was detected only in iGAS emm92-type isolates (representative samples are shown). B) PCR detection of the ermT-gene. The ermT-specific amplicon of 452 bp was detected in emm92 isolates using plasmid DNA as a template. C, D) Detection of the ermA and ermB genes. Chromosomal DNA was used as a template to detect the 612-bp-ermA (C) and 663-bp-ermB (D) amplicons present in several different emm types. A 347-bp-mefA amplicon was detected in a single emm22 isolate (data not shown).

Figure 4. Detection of the methyl transferase genes ermA, ermB, and ermT in invasive group A Streptococcus (iGAS) isolates, West Virginia, USA, 2020–2021. A) Distribution of the pRW35-like plasmid among iGAS isolates. Presence of pRW35-like plasmid DNA was detected only in iGAS emm92-type isolates (representative samples are shown). B) PCR detection of the ermT-gene. The ermT-specific amplicon of 452 bp was detected in emm92 isolates using plasmid DNA as a template. C, D) Detection of the ermA and ermB genes. Chromosomal DNA was used as a template to detect the 612-bp-ermA (C) and 663-bp-ermB (D) amplicons present in several different emm types. A 347-bp-mefA amplicon was detected in a single emm22 isolate (data not shown).

Main Article

1These senior authors contributed equally to this article.

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Page updated: April 14, 2023
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