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Volume 29, Number 6—June 2023
Dispatch

Replication of Novel Zoonotic-Like Influenza A(H3N8) Virus in Ex Vivo Human Bronchus and Lung

Kenrie P.Y. Hui, John C.W. Ho, Ka-Chun Ng, Samuel M.S. Cheng, Ko-Yung Sit, Timmy W.K. Au, Leo L.M. Poon, John M. Nicholls, Malik Peiris, and Michael C.W. ChanComments to Author 
Author affiliations: The University of Hong Kong School of Public Health, Hong Kong, China (K.P.Y. Hui, J.C.W. Ho, K.-C. Ng, S.M.S. Cheng, L.L.M. Poon, M. Peiris, M.C.W. Chan); Centre for Immunology and Infection, Hong Kong (K.P.Y. Hui, J.C.W. Ho, L.L.M. Poon, M. Peiris, M.C.W. Chan); The University of Hong Kong Division of Cardiothoracic Surgery, Hong Kong (K.-Y. Sit, T.W.K. Au); The University of Hong Kong School of Clinical Medicine, Hong Kong (J.M. Nicholls)

Main Article

Figure 1

Comparative replication competence of zoonotic-like influenza A(H3N8) viruses isolated from chicken and other human and avian viruses in ex vivo cultures of human bronchus and lung tissue. Viral M segment RNA copies (A, B) and viral titers (E, F) in culture supernatants were collected at 1, 24, and 48 hours postinfection with H9N2/Y280, pH1N1, avH3N8/MP16, novel H3N8, or H5N1/483 viruses and measured by quantitative reverse transcription PCR (A, B) and TCID50 (E, F). C, D) Viral load from panels A and B by virus strain. G, H) Viral titers from panels E and F by virus strain. Data are geometric mean +SD. Statistical analysis was performed using 2-way (A, B, E, F)or 1-way (C, D, G, H) analysis of variance followed by Tukey posttest; p<0.05 was considered to be statistically significant. Detailed information on viruses used in study is provided in the Appendix. AUC, area under the curve; M, matrix; TCID50, 50% tissue culture infectious dose.

Figure 1. Comparative replication competence of zoonotic-like influenza A(H3N8) viruses isolated from chicken and other human and avian viruses in ex vivo cultures of human bronchus and lung tissue. Viral M segment RNA copies (A, B) and viral titers (E, F) in culture supernatants were collected at 1, 24, and 48 hours postinfection with H9N2/Y280, pH1N1, avH3N8/MP16, novel H3N8, or H5N1/483 viruses and measured by quantitative reverse transcription PCR (A, B) and TCID50 (E, F). C, D) Viral load from panels A and B by virus strain. G, H) Viral titers from panels E and F by virus strain. Data are geometric mean +SD. Statistical analysis was performed using 2-way (A, B, E, F)or 1-way (C, D, G, H) analysis of variance followed by Tukey posttest; p<0.05 was considered to be statistically significant. Detailed information on viruses used in study is provided in the Appendix. AUC, area under the curve; M, matrix; TCID50, 50% tissue culture infectious dose.

Main Article

Page created: March 29, 2023
Page updated: May 17, 2023
Page reviewed: May 17, 2023
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