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Volume 29, Number 8—August 2023
Dispatch

Candidatus Neoehrlichia mikurensis Infection in Patient with Antecedent Hematologic Neoplasm, Spain1

Paola González-Carmona23, Aránzazu Portillo2, Cristina Cervera-Acedo, Daniel González-Fernández, and José A. OteoComments to Author 
Author affiliations: Hospital de Jarrio, Asturias, Spain (P. González-Carmona, D. González-Fernández); Hospital Universitario San Pedro-CIBIR, La Rioja, Spain (A. Portillo, C. Cervera-Acedo, J.A. Oteo)

Main Article

Table 2

Sequence analyses of targeted genes after PCR of DNA from different clinical samples in study of Candidatus Neoehrlichia mikurensis infection in patient with antecedent hematologic neoplasm, Spain*

Disease stage Clinical sample PCR target genes
Panbacteria 16S rRNA 16S rRNA-EHR† groEL‡
Acute
 Bone marrow 98% (13,35/1,362) to 99.8% (1,359/1,362),§ CP054597 100% (306/306), CP054597 99.3% (1,224/1,233), MN701626; 98.9% (1,218/1,232), CP054597
Serum
 95.4% (753/789),§ CP054597
 ND
 99.3% (1,224/1,233), MN701626; 98.9% (1,218/1,232), CP054597
Convalescent, 4th mo
 EDTA blood 98% (1,305/1,332), CP054597 ND ND
Serum
 ND
 ND
 ND
Convalescent, 6th mo EDTA blood ND ND ND
Serum ND ND ND

*Values are highest % identity (identical base pairs/total base pairs), followed by GenBank accession number. ND, not detected. †16S rRNA-EHR refers to the 16S rRNA sequence from the Anaplasmataceae family, which is different from the PCR-amplified panbacteria 16S rRNA sequence. ‡ Percentage of identity with groEL Candidatus N. mikurensis gene from a reference patient in Sweden (GenBank accession no. CP054597) was lower than that of groEL Candidatus N. mikurensis isolate Omsk-41_Micagr from a small mammal in Siberia, Russia (GenBank accession no. MN701626). §Sequence with degenerate bases or obtained from only 1 DNA strand (insufficient sample).

Main Article

1Data from this study were presented at the joint LXIV National Conference of the Spanish Society of Hematology and Hemotherapy, XXXVIII National Conference of the Spanish Society of Thrombosis and Hemostasis, and 38th World Congress of the International Society of Hematology; October 6–8, 2022; Barcelona, Spain; and International Intracellular Bacteria Meeting; August 23–26, 2022; Lausanne, Switzerland.

2These first authors contributed equally to this article.

3Current affiliation: Hospital Universitario San Agustín, Asturias, Spain.

Page created: June 13, 2023
Page updated: July 20, 2023
Page reviewed: July 20, 2023
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