Volume 30, Number 3—March 2024
Research
Microsporidia (Encephalitozoon cuniculi) in Patients with Degenerative Hip and Knee Disease, Czech Republic
, Petra Gottliebová, Elka Nyčová, Nikola Holubová, Jana Fenclová, Marta Kicia, Żaneta Zajączkowska, and Martin Kváč
Table 2
Characteristics of immunocompetent patients and patient samples in study of microsporidia (Encephalitozoon cuniculi) in patients with degenerative hip and knee disease, Czech Republic*
| Patient no. | Age, y/sex | Samples |
|||||
|---|---|---|---|---|---|---|---|
| Origin | Pathology | Total/no. positive† | Genotype‡ | No. spores/g sample (Ct)§ | Microbiology | ||
| 1 |
78/M |
Knee, PR, puncture aspirate, fluid |
PJI |
2/2 |
I |
74 (37) |
Group G beta-hemolytic Streptococcus |
| 29 |
76/M |
Knee, TR, 2 puncture aspirates, fluid, tissue |
PJI |
4/4 |
I |
5,600 (33) |
Enterococcus faecalis |
| 33 |
63/F |
Knee, PR, fluid, tissue |
AIL |
2/2 |
III |
6.9 (39) |
Aseptic |
| 2 |
75/M |
Hip, PR, fluid |
PJI |
1/1 |
II |
260 (35) |
Staphylococcus aureus (MRSA) |
| 7 | 71/F | Hip, PR, fluid, tissue | AIL | 2/2 | I | 12 (38) | Aseptic |
*Samples were collected during May 2020–September 2021 at Bulovka Hospital in Prague, Czech Republic. AIL, aseptic implant loosening; Ct, cycle threshold; MRSA, methicillin-resistant Staphylococcus aureus; PJI, periprosthetic joint infection; PR, primary revision; TR, third and further revision. †Total number of samples/number of E. cuniculi-positive samples. ‡E. cuniculi genotype was determined by nested PCR of partial sequence of the 16S rRNA gene that included the entire internal transcribed spacer. §Number of spores was determined by quantitative real-time PCR of a 268-bp region of the E. cuniculi 16S rRNA gene. DNA was isolated from tissue or aspirate sediment.