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Volume 30, Number 7—July 2024
Dispatch
Body Louse Pathogen Surveillance among Persons Experiencing Homelessness, Canada, 2020–2021
Table 1
Testing of lice to determine species and infection with Bartonella quintana and Acinetobacter spp. from a person experiencing homelessness in inner city Winnipeg, Manitoba, Canada*
Pool | No. lice/pool | Instar | Ct values |
||||
---|---|---|---|---|---|---|---|
Body louse gene | Bartonella ITS3 | B. quintana yopP gene | B. quintana fabB gene | Acinetobacter rpoB | |||
1 | 48 | 1st and 2nd | 31.2 | 33.6 | 30.8 | 30.8 | 40 |
2 | 7 | 1st and 2nd | 29.8 | 35.6 | 36.2 | 35.4 | 40 |
3 | 26 | 3rd | 30.6 | 25.6 | 26.2 | 26.0 | 36.2 |
4 | 26 | 3rd | 30.4 | 23.0 | 27.0 | 26.7 | 38.5 |
5 | 5 | 3rd | 29.9 | 33.6 | 34 | 34.2 | 40 |
6 | 6 | 3rd | 30.5 | 33.3 | 34.2 | 33.8 | 37.4 |
7 | 30 | 4th | 29.3 | 21.8 | 23.1 | 22.9 | 29.6 |
*Ct >40 indicates a negative result. Genes used to determine species identities: PHUM540560, body lice gene distinguishing body lice from head lice; ITS3, internal transcribed spacer 3, identifies Bartonella to genus level; yopP, hypothetical intracellular effector gene, identifies B. quintana to species; fabB, 3-oxoacyl-synthase gene, identifies B. quintana to species; rpoB, RNA polymerase β subunit gene, identifies Acinetobacter spp. Ct, cycle threshold.