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Volume 30, Number 7—July 2024
Dispatch

Body Louse Pathogen Surveillance among Persons Experiencing Homelessness, Canada, 2020–2021

Carl BoodmanComments to Author , Leslie R. Lindsay, Antonia Dibernardo, Kathy Kisil, Heather Coatsworth, Chris Huynh, Amila Heendeniya, John Schellenberg, and Yoav Keynan
Author affiliations: Institute of Tropical Medicine, Antwerp, Belgium (C. Boodman), University of Antwerp, Belgium (C. Boodman); University of Manitoba, Winnipeg, Manitoba, Canada (C. Boodman, A. Heendeniya, J. Schellenberg, Y. Keynan); Public Health Agency of Canada, Winnipeg (L.R. Lindsay, A. Dibernardo, H. Coatsworth, C. Huynh); Access Downtown, Winnipeg (K. Kisil)

Main Article

Table 1

Testing of lice to determine species and infection with Bartonella quintana and Acinetobacter spp. from a person experiencing homelessness in inner city Winnipeg, Manitoba, Canada*

Pool No. lice/pool Instar Ct values
Body louse gene Bartonella ITS3 B. quintana yopP gene B. quintana fabB gene Acinetobacter rpoB
1 48 1st and 2nd 31.2 33.6 30.8 30.8 40
2 7 1st and 2nd 29.8 35.6 36.2 35.4 40
3 26 3rd 30.6 25.6 26.2 26.0 36.2
4 26 3rd 30.4 23.0 27.0 26.7 38.5
5 5 3rd 29.9 33.6 34 34.2 40
6 6 3rd 30.5 33.3 34.2 33.8 37.4
7 30 4th 29.3 21.8 23.1 22.9 29.6

*Ct >40 indicates a negative result. Genes used to determine species identities: PHUM540560, body lice gene distinguishing body lice from head lice; ITS3, internal transcribed spacer 3, identifies Bartonella to genus level; yopP, hypothetical intracellular effector gene, identifies B. quintana to species; fabB, 3-oxoacyl-synthase gene, identifies B. quintana to species; rpoB, RNA polymerase β subunit gene, identifies Acinetobacter spp. Ct, cycle threshold.

Main Article

Page created: May 14, 2024
Page updated: June 22, 2024
Page reviewed: June 22, 2024
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