Volume 30, Number 8—August 2024
Dispatch
Multiplex Dual-Target Reverse Transcription PCR for Subtyping Avian Influenza A(H5) Virus
Malaya K. Sahoo, Ingrid E.A. Morante, ChunHong Huang, Daniel Solis, Fumiko Yamamoto, Uzoamaka C. Ohiri, Daniel Romero, and Benjamin A. Pinsky
Table 3
Non-H5 human and avian influenza A virus genomic RNA used to evaluate H5 specificity in study of multiplex dual-target reverse transcription PCR for subtyping avian influenza A(H5) virus*
| HA gene | Genome | BEI catalog no. |
|---|---|---|
| H1 | A/Brisbane/59/2007 (H1N1) | NR-20080 |
| H1 pdm09 | A/California/04/2009 (H1N1)pdm09 | NR-14689 |
| H2 | A/duck/Germany/1215/1973 (H2N3) | NR-2762 |
| H3 | A/Brisbane/10/2007 (H3N2) | NR-20081 |
| H4 | A/duck/Czechoslovakia/1956 (H4N6) | NR-43012 |
| H6 | A/shearwater/Australia/1/1973 (H6N5) | NR-43014 |
| H8 | A/turkey/Ontario/6118/1968 (H8N4) | NR-43015 |
| H9 | A/turkey/Wisconsin/1/1966 (H9N2) | NR-43016 |
| H10 | A/chicken/Germany/N/1949 (H10N7) | NR-2765 |
| H11 | A/duck/Memphis/546/1974 (H11N9) | NR-43017 |
| H12 | A/duck/Alberta/60/1976 (H12N5) | NR-43018 |
| H13 | A/gull/Maryland/704/1977 (H13N6) | NR-43019 |
| H14 | A/mallard/Astrakhan/263/1982 (H14N5) | NR-43020 |
| H15 | A/shearwater/Australia/2576/1979 (H15N9) | NR-43021 |
| H16 | A/shorebird/Delaware/172/2006 (H16N3) | NR-43022 |
*All genomic RNA obtained from BEI Resources (https://www.beiresources.org). The genomic RNA from these influenza A (H5) viruses was diluted 1:10 in buffer AVE plus carrier RNA (QIAGEN, https://www.qiagen.com). HA, hemagglutinin; pdm09, pandemic 2009.
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