Figure 1. Encounters with dead and live snakes over time from a study of a mortality event in rainbow snakes linked to SFD, United States. A) Map of locations where dead Farancia erytrogramma rainbow snakes were observed during 2013–2023. Exact locations were jittered to obscure sensitive habitat. B) Stacked bar plot showing dead F. erytrogramma snakes found per kilometer in the Back Bay region of Virginia and North Carolina. Dead snakes characterized based on strength of evidence that they died from SFD: suspected, snakes with characteristic SFD lesions in photographs but no screening for Ophidiomyces ophidiicola performed; presumptive, snakes with characteristic SFD lesions, detection of O. ophidiicola, but no necropsies performed; confirmed, snakes with characteristic SFD lesions, detection of O. ophidiicola, and characteristic histologic lesions confirmed through histology; O. ophidiicola detected, snakes with no apparent lesions but tested positive for O. ophidiicola; no lesions, snakes with no apparent lesions in photographs and not tested for O. ophidiicola; unknown, snakes with no photographs of the dorsal and ventral sides. C) Number of live F. erytrogramma snakes encountered in the field per kilometer of transect surveyed with an incorporated 20-coverboard array during 2020–2023 (n = 19; zero-inflated Poisson log-scale year coefficient = −0.482 ± 0.226; p = 0.033) (Appendix Table 3). Total kilometers surveyed per year represented above survey data points. Color shading corresponds to mean infection severity (red is more severe than orange) during sampling event when the species was detected. Gray indicates sampling events without live rainbow snakes detected. SFD, snake fungal disease.