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Volume 31, Number 2—February 2025
Research

Short-Lived Neutralizing Antibody Responses to Monkeypox Virus in Smallpox Vaccine–Naive Persons after JYNNEOS Vaccination

Kara Phipps, Jennifer Yates, Jessica Pettit, Sean Bialosuknia, Danielle Hunt, Alan P. DuPuis, Anne Payne, William Lee, and Kathleen A. McDonoughComments to Author 
Author affiliation: Wadsworth Center, New York State Department of Health, Albany, New York, USA (K. Phipps, J. Yates, J. Pettit, S. Bialosuknia, D. Hunt, A.P. DuPuis II, A. Payne, W. Lee, K.A. McDonough); University at Albany, Albany (J. Yates, K.A. McDonough)

Main Article

Figure 3

Longitudinal neutralizing antibody response by JYNNEOS vaccination extending to 12 months in a study to assess neutralizing antibody responses to MPXV in smallpox vaccine–naive persons after JYNNEOS vaccination. We used PRNT to test serum samples from donors vaccinated with 2 doses of JYNNEOS ≈28 days apart. We performed assays with sonicated virus and a 1-hour virus–serum incubation. A) MPXV PRNT50 results; B) VACV PRNT50 results; C) MPXV PRNT90 results; D) VACV PRNT90 results. Data from a single donor with prior smallpox vaccination are plotted separately (black Xs). We used data from participants with no known vaccinia exposure for mean calculations (black circles). Each datapoint represents the geometric mean titer of 2 independent experiments, each performed in duplicate, and the limits of detection are expressed by horizontal dotted lines. MPXV, monkeypox virus; prevax, prevaccination; PRNT, plaque reduction neutralization test; PRNT50, 50% plaque reduction as measured by PRNT; PRNT90, 90% plaque reduction as measured by PRNT; VACV, vaccinia virus.

Figure 3. Longitudinal neutralizing antibody response by JYNNEOS vaccination extending to 12 months in a study to assess neutralizing antibody responses to MPXV in smallpox vaccine–naive persons after JYNNEOS vaccination. We used PRNT to test serum samples from donors vaccinated with 2 doses of JYNNEOS ≈28 days apart. We performed assays with sonicated virus and a 1-hour virus–serum incubation. A) MPXV PRNT50 results; B) VACV PRNT50 results; C) MPXV PRNT90 results; D) VACV PRNT90 results. Data from a single donor with prior smallpox vaccination are plotted separately (black Xs). We used data from participants with no known vaccinia exposure for mean calculations (black circles). Each datapoint represents the geometric mean titer of 2 independent experiments, each performed in duplicate, and the limits of detection are expressed by horizontal dotted lines. MPXV, monkeypox virus; prevax, prevaccination; PRNT, plaque reduction neutralization test; PRNT50, 50% plaque reduction as measured by PRNT; PRNT90, 90% plaque reduction as measured by PRNT; VACV, vaccinia virus.

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Page created: December 11, 2024
Page updated: January 10, 2025
Page reviewed: January 10, 2025
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