Table 2

Disclaimer: Early release articles are not considered as final versions. Any changes will be reflected in the online version in the month the article is officially released.

Carbapenem-Resistant, Virulence Plasmid–Harboring Klebsiella pneumoniae, United States

Jianping Jiang, Tengfei Long, Adeline R. Porter, Arianne Lovey, Annie Lee, Jesse Thomas Jacob, Cesar A. Arias, Robert Bonomo, Robert Kalayjian, Yanan Zhao, Frank R. DeLeo, David van Duin, Barry N. Kreiswirth

, and Liang Chen

Author affiliation: Hackensack-Meridian Health Center for Discovery and Innovation, Nutley, New Jersey, USA (J. Jiang, T. Long, A. Lovey, A. Lee, Y. Zhao, B.N. Kreiswirth, L. Chen); National Institute of Allergy and Infectious Disease Rocky Mountain Laboratories, Hamilton, Montana, USA (A.R. Porter, F.R. DeLeo); Emory University, Atlanta, Georgia, USA (J.T. Jacob); Houston Methodist Hospital and Houston Methodist Research Institute, Houston, Texas, USA (C.A. Arias); Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio, USA (R.A. Bonomo); Case Western Reserve University School of Medicine, Cleveland (R.A. Bonomo); MetroHealth Medical Center, Cleveland (R. Kalayjian); University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA (D. van Duin)

Main Article

Genomic features of isolates in study of carbapenem-resistant, virulence plasmid–harboring Klebsiella pneumoniae isolates, United States*

Genomic feature	Isolate
Genomic feature	ARLG-4622	ARLG-3254	ARLG-4744	ARLG-7683	ARLG-3484	ARLG-4720
Genome size, Mb	5.6	5.8	5.9	5.9	5.8	5.8
Plasmid number	3	3	5	5	5	5
MLST	ST23	ST23	ST11	ST11	ST893	ST893
K locus type	KL1	KL1	KL64	KL64	KL20	KL20
O locus type	O1/O2v2	O1/O2v2	O1/O2v1	O1/O2v1	O3/O3a	O3/O3a
ESBL or carbapenemase plasmids
ESBL or (and)carbapenemase	KPC-2	KPC-2	CTX-M-65	CTX-M-65	CTX-M-15, OXA-48	CTX-M-15, OXA-48
Size, bp	107766	142552	106012	124552	185494	185434
Replicon	repA_pKPC-2	IncFIB(pQil)/IncFII	IncFII(pHN7A8)/IncR	IncFII/pHN7A8/IncR	IncL/M/IncFII/IncFIA(HI1)	IncL/M/IncFII/IncFIA(HI1)
OmpK35/OmpK36†	WT/WT	WT/WT	OmpK35(IS)/OmpK36GD	OmpK35(IS)/OmpK36GD	OmpK35(IS)/OmpK36(IS)	OmpK35(IS)/OmpK36(IS)
Other acquired AMR genes	bla_TEM-1D	aac (3)-IIa; ant(2”)-Ia; catB3; sul1; dfrA14; bla_TEM-1D	aadA2; rmtB; fosA3; qnrS1; sul2; tet(A); dfrA14; bla_TEM-1D; bla_LAP-2	aadA2; rmtB; fosA3; qnrS1; sul2; tet(A); dfrA14; bla_TEM-1D; bla_LAP-2	aac(6')-Ib-cr; strA; strB; qnrB1; catB4; sul2; tet(A); dfrA14; bla_TEM-1D; bla_OXA-1	aac(6')-Ib-cr; strA; strB; qnrB1; catB4; sul2; tet(A); dfrA14; bla_TEM-1D; bla_OXA-1
Other AMR-related mutations	GyrA-87G	None	GyrA-83I, 87G; ParC-80I	GyrA-83I,87G; ParC-80I	MgrB-62% (frameshift); GyrA-83F	MgrB-62% (frameshift); GyrA-83F
Hypervirulent plasmid size (BLASTn identity, coverage to pK2044)	229.8Kb (99%, 97%)	223.7Kb (99%, 98%)	217.8Kb (99%, 92%)	218.6Kb (99%, 92%)	164.4Kb (99%, 67%)	185.4Kb (99%, 67%)
Virulence genes	iuc, iro, rmpADC(ΔrmpAC), ΔrmpA2, Δpeg-344, ybt, clb	iuc, iro, rmpADC, ΔrmpA2, peg-344, ybt, clb	iuc, Δiro, rmpADC, ΔrmpA2, peg-344, ybt	iuc, Δiro, rmpADC(ΔrmpA), ΔrmpA2, peg-344, ybt	iuc, ΔrmpA2, ybt	iuc, ΔrmpA2, ybt
CPS mutation	Δugd	ΔcpsACP	None	None	None	None

*AMR, antimicrobial resistance; CPS, capsule polysaccharide; ESBL, extended-spectrum β-lactamase; MLST, multilocus sequence type; ST, sequence type; WT, wild-type. †OmpK35(IS), OmpK35 interrupted by insertion sequence; OmpK36GD, OmpK36 Gly134-Asp135 (GD) insertion variant.

Main Article

Page created: February 18, 2025

Page updated: March 11, 2025

Page reviewed: March 11, 2025

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