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Volume 31, Number 5—May 2025

Dispatch

Influenza A(H1N1)pdm09 Virus with Reduced Susceptibility to Baloxavir, Japan, 2024

Emi TakashitaComments to Author , Hiroko Morita, Shiho Nagata, Seiichiro Fujisaki, Hideka Miura, Tatsuya Ikeda, Kenichi Komabayashi, Mika Sasaki, Yohei Matoba, Tomoko Takahashi, Naomi Ogawa, Katsumi Mizuta, Sueshi Ito, Noriko Kishida, Kazuya Nakamura, Masayuki Shirakura, Shinji Watanabe, and Hideki Hasegawa
Author affiliation: Author affiliations: National Institute of Infectious Diseases, Tokyo, Japan (E. Takashita, H. Morita, S. Nagata, S. Fujisaki, H. Miura, N. Kishida, K. Nakamura, M. Shirakura, S. Watanabe, H. Hasegawa); Yamagata Prefectural Institute of Public Health, Yamagata, Japan (T. Ikeda, K. Komabayashi, M. Sasaki, Y. Matoba, T. Takahashi, N. Ogawa, K. Mizuta); Ito Clinic, Yamagata (S. Ito)

Main Article

Figure 2

In vitro growth kinetics of the polymerase acidic I38N mutation from study of influenza A(H1N1)pdm09 virus with reduced susceptibility to baloxavir, Japan, 2024. Humanized MDCK cells were infected with the polymerase acidic I38N mutant virus (A/Yamagata/103/2024) or its corresponding wild-type virus (A/Yamagata/336/2023) at a multiplicity of infection of 0.001 focus-forming units per cell. The supernatants were harvested at the indicated times and virus was titrated by using a focus assay. Means (circles) and SDs (error bars) of 4 experiments are shown. p values were calculated by using a t-test and fitting a mixed-effects model. FFU, focus-forming units; PA, polymerase acidic.

Figure 2. In vitro growth kinetics of the polymerase acidic I38N mutation from study of influenza A(H1N1)pdm09 virus with reduced susceptibility to baloxavir, Japan, 2024. Humanized MDCK cells were infected with the polymerase acidic I38N mutant virus (A/Yamagata/103/2024) or its corresponding wild-type virus (A/Yamagata/336/2023) at a multiplicity of infection of 0.001 focus-forming units per cell. The supernatants were harvested at the indicated times and virus was titrated by using a focus assay. Means (circles) and SDs (error bars) of 4 experiments are shown. p values were calculated by using a t-test and fitting a mixed-effects model. FFU, focus-forming units; PA, polymerase acidic.

Main Article

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