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Volume 31, Number 6—June 2025

Research

Diagnostic Accuracy of 3 Mpox Lateral Flow Assays for Antigen Detection, Democratic Republic of the Congo and United Kingdom

Elie Ishara-Nshombo1Comments to Author , Anushri Somasundaran1, Alessandra Romero-Ramirez, Konstantina Kontogianni, Daniel Mukadi-Bamuleka, Marithé Mukoka-Ntumba, Emile Muhindo-Milonde, Hugues Mirimo-Nguee, Jacob Parkes, Yusra Hussain, Susan Gould, Christopher T. Williams, Dominic Wooding, Juvenal Nkeramahame, Mikaela Watson, Hayley E. Hardwick, Malcolm G. Semple, John Kenneth Baillie, Jake Dunning, Thomas E. Fletcher, Thomas Edwards, Devy M. Emperador, Hugo Kavunga-Membo1, Ana Isabel Cubas-Atienzar1Comments to Author , and ISARIC 4 and C Investigators,2
Author affiliation: Rodolphe Mérieux Laboratory, Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo (E. Ishara-Nshombo, D. Mukadi-Bamuleka, M. Mukoka-Ntumba, E. Muhindo-Milonde, H. Mirimo-Nguee, H. Kavunga-Membo); Liverpool School of Tropical Medicine, Liverpool, UK (A. Somasundaran, A. Romero-Ramirez, K. Kontogianni, J. Parkes, Y. Hussain, S. Gould, C.T. Williams, D. Wooding, T.E. Fletcher, T. Edwards, A. Cubas-Atienzar); Royal Liverpool University Hospital NHS Foundation Trust, Liverpool (S. Gould, T.E. Fletcher); Foundation for Innovative New Diagnostics, Geneva, Switzerland (J. Nkeramahame, M. Watson, D.M. Emperador); University of Liverpool, Liverpool (H.E. Hardwick, M.G. Semple); University of Edinburgh, Edinburgh, Scotland, UK (J.K. Baillie); Pandemic Sciences Institute, University of Oxford, Oxford, UK (J. Dunning); Royal Free London NHS Foundation Trust, London, UK (J. Dunning)

Main Article

Figure 1

Boxplots of Ct values from paired URS and SS tested by Sansure quantitative PCR in study of diagnostic accuracy of 3 mpox lateral flow assays for antigen detection, Democratic Republic of the Congo (DRC) and United Kingdom. A) DRC, n = 14; B) United Kingdom, n = 9. Horizontal lines within boxes indicate medians, box tops and bottoms indicate interquartile range, and whiskers indicate maximum and minimum values. Ct values were significantly higher (p<0.05) in the URS group than in the SS group in the DRC cohort. Ct, cycle threshold; SS, skin lesion samples; URS, upper respiratory specimens.

Figure 1. Boxplots of Ct values from paired URS and SS tested by Sansure quantitative PCR in study of diagnostic accuracy of 3 mpox lateral flow assays for antigen detection, Democratic Republic of the Congo (DRC) and United Kingdom. A) DRC, n = 14; B) United Kingdom, n = 9. Horizontal lines within boxes indicate medians, box tops and bottoms indicate interquartile range, and whiskers indicate maximum and minimum values. Ct values were significantly higher (p<0.05) in the URS group than in the SS group in the DRC cohort. Ct, cycle threshold; SS, skin lesion samples; URS, upper respiratory specimens.

Main Article

1These authors contributed equally to this article.

2Members of ISARIC 4C investigator group are listed at the end of this article.

Page created: April 08, 2025
Page updated: May 27, 2025
Page reviewed: May 27, 2025
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