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Volume 31, Number 6—June 2025

Research

Diagnostic Accuracy of 3 Mpox Lateral Flow Assays for Antigen Detection, Democratic Republic of the Congo and United Kingdom

Elie Ishara-Nshombo1Comments to Author , Anushri Somasundaran1, Alessandra Romero-Ramirez, Konstantina Kontogianni, Daniel Mukadi-Bamuleka, Marithé Mukoka-Ntumba, Emile Muhindo-Milonde, Hugues Mirimo-Nguee, Jacob Parkes, Yusra Hussain, Susan Gould, Christopher T. Williams, Dominic Wooding, Juvenal Nkeramahame, Mikaela Watson, Hayley E. Hardwick, Malcolm G. Semple, John Kenneth Baillie, Jake Dunning, Thomas E. Fletcher, Thomas Edwards, Devy M. Emperador, Hugo Kavunga-Membo1, Ana Isabel Cubas-Atienzar1Comments to Author , and ISARIC 4 and C Investigators,2
Author affiliation: Rodolphe Mérieux Laboratory, Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo (E. Ishara-Nshombo, D. Mukadi-Bamuleka, M. Mukoka-Ntumba, E. Muhindo-Milonde, H. Mirimo-Nguee, H. Kavunga-Membo); Liverpool School of Tropical Medicine, Liverpool, UK (A. Somasundaran, A. Romero-Ramirez, K. Kontogianni, J. Parkes, Y. Hussain, S. Gould, C.T. Williams, D. Wooding, T.E. Fletcher, T. Edwards, A. Cubas-Atienzar); Royal Liverpool University Hospital NHS Foundation Trust, Liverpool (S. Gould, T.E. Fletcher); Foundation for Innovative New Diagnostics, Geneva, Switzerland (J. Nkeramahame, M. Watson, D.M. Emperador); University of Liverpool, Liverpool (H.E. Hardwick, M.G. Semple); University of Edinburgh, Edinburgh, Scotland, UK (J.K. Baillie); Pandemic Sciences Institute, University of Oxford, Oxford, UK (J. Dunning); Royal Free London NHS Foundation Trust, London, UK (J. Dunning)

Main Article

Figure 2

Number of positive and negative results by test and by days from symptom onset in study of diagnostic accuracy of 3 mpox lateral flow assays for antigen detection, Democratic Republic of the Congo (DRC) and United Kingdom. A) Skin lesion swab samples in DRC; B) upper respiratory swab samples in DRC; C) skin swab samples in the United Kingdom; D) upper respiratory swab samples in the United Kingdom. Rapid diagnostic tests evaluated: Ecotest, Ecotest Monkeypox Antigen Rapid Test (Assure Tech [Hangzhou] Co., Ltd., https://www.assuretech-product.com); Flowflex, FlowFlex Monkeypox Virus Antigen Rapid Test (ACON Biotech [Hangzhou] Co., Ltd., https://www.aconbio.com); Standard Q, Standard Q Monkeypox Ag Test (SD Biosensor, https://www.sdbiosensor.com). Sansure qPCR (Monkeypox Virus Nucleic Acid Diagnostic Kit; Sansure Biotech, https://www.sansureglobal.com) was used to evaluate results of the rapid diagnostic tests.

Figure 2. Number of positive and negative results by test and by days from symptom onset in study of diagnostic accuracy of 3 mpox lateral flow assays for antigen detection, Democratic Republic of the Congo (DRC) and United Kingdom. A) Skin lesion swab samples in DRC; B) upper respiratory swab samples in DRC; C) skin swab samples in the United Kingdom; D) upper respiratory swab samples in the United Kingdom. Rapid diagnostic tests evaluated: Ecotest, Ecotest Monkeypox Antigen Rapid Test (Assure Tech [Hangzhou] Co., Ltd., https://www.assuretech-product.com); Flowflex, FlowFlex Monkeypox Virus Antigen Rapid Test (ACON Biotech [Hangzhou] Co., Ltd., https://www.aconbio.com); Standard Q, Standard Q Monkeypox Ag Test (SD Biosensor, https://www.sdbiosensor.com). Sansure qPCR (Monkeypox Virus Nucleic Acid Diagnostic Kit; Sansure Biotech, https://www.sansureglobal.com) was used to evaluate results of the rapid diagnostic tests.

Main Article

1These authors contributed equally to this article.

2Members of ISARIC 4C investigator group are listed at the end of this article.

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Page updated: May 02, 2025
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