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Diagnostic Accuracy of 3 Mpox Lateral Flow Assays for Antigen Detection, Democratic Republic of the Congo and United Kingdom
Elie Ishara-Nshombo
1
, Anushri Somasundaran
1, Alessandra Romero-Ramirez, Konstantina Kontogianni, Daniel Mukadi-Bamuleka, Marithé Mukoka-Ntumba, Emile Muhindo-Milonde, Hugues Mirimo-Nguee, Jacob Parkes, Yusra Hussain, Susan Gould, Christopher T. Williams, Dominic Wooding, Juvenal Nkeramahame, Mikaela Watson, Hayley E. Hardwick, Malcolm G. Semple, John Kenneth Baillie, Jake Dunning, Thomas E. Fletcher, Thomas Edwards, Devy M. Emperador, Hugo Kavunga-Membo
1, Ana Isabel Cubas-Atienzar
1
, and
ISARIC 4 and
C Investigators,2
Author affiliation: Rodolphe Mérieux Laboratory, Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo (E. Ishara-Nshombo, D. Mukadi-Bamuleka, M. Mukoka-Ntumba, E. Muhindo-Milonde, H. Mirimo-Nguee, H. Kavunga-Membo); Liverpool School of Tropical Medicine, Liverpool, UK (A. Somasundaran, A. Romero-Ramirez, K. Kontogianni, J. Parkes, Y. Hussain, S. Gould, C.T. Williams, D. Wooding, T.E. Fletcher, T. Edwards, A. Cubas-Atienzar); Royal Liverpool University Hospital NHS Foundation Trust, Liverpool (S. Gould, T.E. Fletcher); Foundation for Innovative New Diagnostics, Geneva, Switzerland (J. Nkeramahame, M. Watson, D.M. Emperador); University of Liverpool, Liverpool (H.E. Hardwick, M.G. Semple); University of Edinburgh, Edinburgh, Scotland, UK (J.K. Baillie); Pandemic Sciences Institute, University of Oxford, Oxford, UK (J. Dunning); Royal Free London NHS Foundation Trust, London, UK (J. Dunning)
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Figure 2
![Number of positive and negative results by test and by days from symptom onset in study of diagnostic accuracy of 3 mpox lateral flow assays for antigen detection, Democratic Republic of the Congo (DRC) and United Kingdom. A) Skin lesion swab samples in DRC; B) upper respiratory swab samples in DRC; C) skin swab samples in the United Kingdom; D) upper respiratory swab samples in the United Kingdom. Rapid diagnostic tests evaluated: Ecotest, Ecotest Monkeypox Antigen Rapid Test (Assure Tech [Hangzhou] Co., Ltd., https://www.assuretech-product.com); Flowflex, FlowFlex Monkeypox Virus Antigen Rapid Test (ACON Biotech [Hangzhou] Co., Ltd., https://www.aconbio.com); Standard Q, Standard Q Monkeypox Ag Test (SD Biosensor, https://www.sdbiosensor.com). Sansure qPCR (Monkeypox Virus Nucleic Acid Diagnostic Kit; Sansure Biotech, https://www.sansureglobal.com) was used to evaluate results of the rapid diagnostic tests.](/eid/images/25-0166-F2.jpg)
Figure 2. Number of positive and negative results by test and by days from symptom onset in study of diagnostic accuracy of 3 mpox lateral flow assays for antigen detection, Democratic Republic of the Congo (DRC) and United Kingdom. A) Skin lesion swab samples in DRC; B) upper respiratory swab samples in DRC; C) skin swab samples in the United Kingdom; D) upper respiratory swab samples in the United Kingdom. Rapid diagnostic tests evaluated: Ecotest, Ecotest Monkeypox Antigen Rapid Test (Assure Tech [Hangzhou] Co., Ltd., https://www.assuretech-product.com); Flowflex, FlowFlex Monkeypox Virus Antigen Rapid Test (ACON Biotech [Hangzhou] Co., Ltd., https://www.aconbio.com); Standard Q, Standard Q Monkeypox Ag Test (SD Biosensor, https://www.sdbiosensor.com). Sansure qPCR (Monkeypox Virus Nucleic Acid Diagnostic Kit; Sansure Biotech, https://www.sansureglobal.com) was used to evaluate results of the rapid diagnostic tests.
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