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Volume 31, Number 9—September 2025

Research

Detection of Multiple Nosocomial Trichosporon asahii Transmission Events via Microsatellite Typing Assay, South America

Elaine C. Francisco, Norma B. Fernández, Mauricio Carbia, Chendo Dieleman, Andra-Cristina Bostanaru-Iliescu, Jos Houbraken, Arnaldo L. Colombo, and Ferry HagenComments to Author 
Author affiliation: Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands (E.C. Fancisco, C. Dieleman, J. Houbraken, F. Hagen); Escola Paulista de Medicina-Universidade Federal de São Paulo, São Paulo, Brazil (E.C. Francisco, A.L. Colombo); Hospital de Clínicas José de San Martín, Universidad de Buenos Aires, Buenos Aires, Argentina (N.B. Fernández); Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay (M. Carbia); Iași University of Life Sciences, Iasi, Romania (A.-C. Bostanaru-Iliescu); Antimicrobial Resistance Institute of São Paulo, São Paulo (A.L. Colombo); University Medical Center Utrecht, Utrecht (F. Hagen); Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, the Netherlands (F. Hagen)

Main Article

Table

Microsatellite typing panel used for detection of multiple nosocomial Trichosporon asahii transmission events via microsatellite typing assay, South America*

Accession no. Contig; reference code† Expected size; total fragment, bp‡ Repeat unit D value; no. alleles Forward primer, 5′ → 3′ Reverse primer, 5′ → 3′
CP116786
 6; E
 173; 206
 CA
 0.8280; 12
 FLU-TCGTCTGTCATCGACCCATA
 GGCTCAGCTGAAGCTCACTT
CP116785
 5; G
 123; 153
 GA
 0.7731; 11
 FLU-TCCCTTTGATTTGGGTGTGT
 CTCTCCCAGGTTCGTTTCAA
CP116781
 1; I
 183; 211
 TG
 0.7481; 9
 FLU-AGCCTTAGTTGCCCTTGTCA
 ACTCAACACTTGGGCGACTT
CP116782
 2; K
 139; 165
 AG
 0.7666; 10
 GATCGAGTCCAAGGAACGAC
 FLU-TTCCCGTCCACCTTTACTGA
CP116785
 5; P
 112; 158
 TCGT
 0.6600; 14
 FLU-ACGAACTCCATGGCTGAGTC
 TGACTGACAACACACCCGATA
CP116785 5; Q 135; 169 GTT 0.6452; 12 FLU-ATCTCGGTTGTTGCCGTTAT GCAACAGCAACAGCAGTACC

*Isolates sourced from CBS culture collection (https://wi.knaw.nl/fungal_table) hosted by the Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands. Discriminatory power of microsatellite typing panel assay as determined via the Simpson index of diversity (D). FLU, fluorescein. †de novo assembly contig number; microsatellite panel reference code. ‡The expected fragment size without the repeat units; number of alleles refers to the total fragment size of the T. asahii type strain CBS 2479.

Main Article

Page created: June 18, 2025
Page updated: August 18, 2025
Page reviewed: August 18, 2025
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