Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link

Disclaimer: Early release articles are not considered as final versions. Any changes will be reflected in the online version in the month the article is officially released.

Volume 31, Number 9—September 2025

Dispatch

Imported Malaria and Congenital Acquisition in Infant, Portugal, 2024

Inês Lopes, Joana Dias, Edvaldo Das Neves, Maria Morais, Ana Santos-Reis, Ana M. Garcia, Luis Varandas1, and Dinora Lopes1Comments to Author 
Author affiliation: Global Health and Tropical Medicine, Associate Laboratory in Translation and Innovation Towards Global Health, Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa, Lisbon, Portugal (I. Lopes, E. Das Neves, A. Santos-Reis, L. Varandas, D. Lopes); Unidade Local de Saúde São José, Lisbon (J. Dias); Hospital of Vila Franca de Xira, Vila Franca de Xira, Portugal (M. Morais); Unidade Local de Saúde São José EPE Centro de Investigação, Lisbon (A.M. Garcia, L. Varandas)

Main Article

Table 1

PCR amplification of Plasmodium spp. in study of malaria in mother and infant, Portugal*

Target
 Primers
 Assay type
 Amplification conditions
18ssrRNA
Plasmodium sp. 1st PCR reaction: rPLU forward primer, 5′-CTTGTTGTTGCCTTAAACTTC-3′; rPLU reverse primer, 5′-TTAAAATTGTTGCAGTTAAAACG-3′ Nested PCR 1× PCR master mix,† T1 Thermocycler–Biometic: 1 cycle, 95°C, 3 min); 30 cycles, 94°C, 1 min; 58°C, 1 min; 72°C, 1 min
P. falciparum
 Nested PCR: Pf forward primer, 5′-TTAAACTGGTTTGGGAAAACCAAATATATT-3′; Pf reverse primer, 5′-ACACAATGAACTCAATCATGACTACCCGTC-3′ (Singh et al. [8])
1× PCR master mix,† 1 cycle, 95°C, 3 min; 35 cycles, 94°C, 1 min; 58°C, 1 min; 72°C, 1 min
varATS Forward primer, 5′-CCCATACACAACCAAYTGGA-3′; reverse primer, 5′-TTCGCACATATCTCTATGTCTATCT-3′; probe, 5′-6-FAM-TRTTCCATAAATGGT-NFQ-MGB-3′
(Hofmann et al. [6])		 qPCR 1× TaqMan Gene Expression Mastermix,† 0.8 μM of each primer, 0.4 μM of probe; CFX96 Real-Time PCR Detection System‡: 1 cycle, 50°C, 2 min; 95°C 10 min; 45 cycles, 95°C, 15 sec; 55°C, 1 min
dPCR 5x Absolute Q DNA Digital PCR Mastermix;§ Absolute Q Digital PCR§: 1 cycle, 50°C, 2 min; 95°C 10 min; 45 cycles, 95°C, 15 sec; 55°C, 1 min

*dPCR, digital PCR; qPCR, quantitative PCR. †Thermo Fisher Scientific, https://www.thermofisher.com. ‡Bio-Rad Laboratories, https://www.bio-rad.com. §Applied Biosystems, https://www.thermofisher.com

Main Article

1These authors contributed equally to this article.

Page created: August 01, 2025
Page updated: August 20, 2025
Page reviewed: August 20, 2025
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external