Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link

Disclaimer: Early release articles are not considered as final versions. Any changes will be reflected in the online version in the month the article is officially released.

Volume 32, Number 12—December 2026

Dispatch

Natural Occurrence of Primate SLAM-Adapted Canine Distemper Virus, Brazil, 2018

Mizuki Tominaga, Ayumu Hyodo, Shohei Kojima, Mitsuki Onodera, Hekai Yang, Fumio Seki, Yuki Kitai, Yukiko Akahori, Hiroshi Katoh, and Makoto TakedaComments to Author 
Author affiliation: The University of Tokyo, Bunkyo-ku, Tokyo, Japan (M. Tominaga, A. Hyodo, M. Onodera, H. Yang, Y. Kitai, Y. Akahori, H. Katoh, M. Takeda); Keio University Human Biology-Microbiome-Quantum Research Center, Tokyo (S. Kojima); Okayama University Medical School, Okayama, Japan (M. Onodera); Japan Institute of Health Security, Musashimurayama, Japan (F. Seki)

Main Article

Figure 2

Cell fusion assay in study of natural occurrence of primate SLAM-adapted canine distemper virus, Brazil, 2018. Vero cells constitutively expressing marmoset (GenBank accession no. XM_002760176), canine (accession no. AF325357), human (accession no. NM_003037.4), or macaque (accession no. XM_001117605.3) SLAM, as well as parental Vero cells, were transfected with pCAGGS plasmids (RIKEN BioResource Research Center, https://dnaconda.riken.jp) expressing both canine distemper virus fusion and hemagglutinin (HA) proteins. HA genes were derived from the Ac96I strain (accession no. AB753775) (A) or NHP1 strain (accession no. PP847352.1) (B). Fusion gene was derived from the Ac96I virus strain. Mutations were introduced at positions 519 and 530 of the HA genes, and amino acid substitutions are indicated. Syncytial areas showing cell–cell fusion are highlighted in green. Images represent 3 independent experiments. Original magnification ×100.

Figure 2. Cell fusion assay in study of natural occurrence of primate SLAM-adapted canine distemper virus, Brazil, 2018. Vero cells constitutively expressing marmoset (GenBank accession no. XM_002760176), canine (accession no. AF325357), human (accession no. NM_003037.4), or macaque (accession no. XM_001117605.3) SLAM, as well as parental Vero cells, were transfected with pCAGGS plasmids (RIKEN BioResource Research Center, https://dnaconda.riken.jp) expressing both canine distemper virus fusion and hemagglutinin (HA) proteins. HA genes were derived from the Ac96I strain (accession no. AB753775) (A) or NHP1 strain (accession no. PP847352.1) (B). Fusion gene was derived from the Ac96I virus strain. Mutations were introduced at positions 519 and 530 of the HA genes, and amino acid substitutions are indicated. Syncytial areas showing cell–cell fusion are highlighted in green. Images represent 3 independent experiments. Original magnification ×100.

Main Article

Page created: September 16, 2026
Page updated: October 27, 2026
Page reviewed: October 27, 2026
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external